Detailed information NTG0575015

Bioinformatically predicted

Overview

• Name: comB4
• Type: Machinery gene
• Locus tag: MPF92_RS06540
• Genome accession: NZ_CP094106
• Coordinates: 1371780..1374149 (+)
• Length: 789 a.a.
• NCBI ID: WP_245023721.1
• Uniprot ID: -
• Organism: Helicobacter pylori strain Hpfe075
• Function: transformation-associated type IV transport system (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1366780..1379149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MPF92_RS06495 (MPF92_06495)	-	1367494..1367802 (-)	309	WP_245024488.1	DUF1104 domain-containing protein	-
MPF92_RS06500 (MPF92_06500)	-	1367741..1368143 (+)	403	Protein_1263	P-type conjugative transfer protein TrbL	-
MPF92_RS06505 (MPF92_06505)	-	1368216..1368486 (+)	271	Protein_1264	CAAX protease	-
MPF92_RS06510 (MPF92_06510)	-	1368541..1369322 (+)	782	Protein_1265	hypothetical protein	-
MPF92_RS06515 (MPF92_06515)	-	1369342..1369635 (+)	294	WP_245023717.1	hypothetical protein	-
MPF92_RS06520 (MPF92_06520)	-	1369642..1371168 (+)	1527	Protein_1267	hypothetical protein	-
MPF92_RS06530 (MPF92_06530)	-	1371248..1371466 (+)	219	WP_245023720.1	virB2 type IV secretion protein	-
MPF92_RS06535 (MPF92_06535)	comB3	1371467..1371733 (+)	267	WP_154415622.1	virB3 type IV secretion protein	Machinery gene
MPF92_RS06540 (MPF92_06540)	comB4	1371780..1374149 (+)	2370	WP_245023721.1	VirB4 family type IV secretion/conjugal transfer ATPase	Machinery gene
MPF92_RS07935 (MPF92_06550)	-	1374152..1375785 (+)	1634	Protein_1271	type IA DNA topoisomerase	-
MPF92_RS06555 (MPF92_06555)	-	1375748..1375936 (-)	189	Protein_1272	DUF1104 domain-containing protein	-
MPF92_RS06560 (MPF92_06560)	-	1376290..1377150 (-)	861	WP_000750190.1	outer membrane protein	-
MPF92_RS06565 (MPF92_06565)	rplT	1377349..1377699 (-)	351	WP_001264172.1	50S ribosomal protein L20	-
MPF92_RS06570 (MPF92_06570)	rpmI	1377792..1377986 (-)	195	WP_001125542.1	50S ribosomal protein L35	-
MPF92_RS06575 (MPF92_06575)	infC	1377967..1378578 (-)	612	WP_000089219.1	translation initiation factor IF-3	-

Sequence

Protein

• Length: 789 a.a.
• Molecular weight: 91184.55 Da
• Isoelectric Point: 7.5315

>NTDB_id=575015 MPF92_RS06540 WP_245023721.1 1371780..1374149(+) (comB4) [Helicobacter pylori strain Hpfe075]
MKEETNIVGIYNEHYLLSEKSNLVGALKLEGISYLSLDDKEIAQKFNERILALNEIVDGVHFKIVAKRRKIFMHHEYTQE
EISNPFALEVINLWEQGVEDVYQNFYYLIFETKNDSIKGYLERFKKKITTNELENIQENNKQDEVKYQENYFIGFDNFNL
LDKSKILDSIINNVKNMLSGLFVEKIDANSLLNFYAEYINGVPSEYTFARGRLHDGMINSDVHFKKDFFTHIHNSKEIFK
RFISIKTYDIDKIVSTALSSVLHLFLEFDVILNIDNIPKAKAEKRIETKRKRANKSIRVEIDELNDMIKTDRVLMQEISL
NILVHAKTKTDLDSACIEITNLLKQKGIVSAQESIGMLPMFFSFFPNRNRLNFRKRLLSSQNIASLIILEKQNCGFSRNS
WGDRHLTIFRNQDKSPYLFNFHAYEAKRKNDMVSGHTIIFGGTGAGKTTLIEFLITNCFKYEDLSILALDRNNGMRVMTE
FLDGQYNDSNDFYINPFSLKDTSANCTFLVSWLAFMLNIDEDTKDEKEKKTLSALSKTIRVCYNNITKQGGAIFKLRDFK
DTLERDYLDSKDILEKEGSKDLYKHSTDCLDFAKKLSVIDMDALSSNKKDFNLAVPYLFYKVMNRAKLENKPFYIFIDEA
KSVIENPIMLAKIKDTLAQARKLNGVLTLAFQDINQLDGVEGAKSIIENAAQVILYPTNNFEKLESYGILLSPIEKSFLN
KTPLNARQVLVKNLLTQSSVFLEINLEKLNSTSKNFLRAYSSSASSVFELETLKKDNPKNYKEIYLTKE

Nucleotide

• Length: 2370 bp

>NTDB_id=575015 MPF92_RS06540 WP_245023721.1 1371780..1374149(+) (comB4) [Helicobacter pylori strain Hpfe075]
ATGAAAGAAGAAACTAACATTGTAGGCATTTACAATGAGCATTATCTTTTAAGTGAAAAATCAAACCTTGTAGGCGCTCT
TAAGTTAGAGGGAATTTCTTATTTGTCTTTAGATGACAAAGAAATAGCGCAAAAATTTAATGAACGCATTTTGGCTCTTA
ATGAGATTGTAGATGGAGTGCATTTTAAAATCGTAGCTAAAAGGCGTAAGATTTTCATGCACCATGAATACACTCAAGAG
GAGATAAGCAATCCTTTTGCATTAGAGGTTATCAATTTATGGGAGCAAGGGGTTGAGGATGTTTATCAAAATTTCTATTA
TTTAATTTTTGAAACTAAGAATGATAGCATTAAAGGTTATTTGGAACGATTTAAGAAAAAAATCACTACCAATGAATTAG
AAAATATTCAAGAAAATAATAAACAAGATGAAGTTAAATATCAAGAAAACTATTTCATAGGCTTTGATAATTTTAATCTA
TTAGATAAATCTAAAATATTAGACAGCATTATCAATAATGTTAAAAACATGCTTTCTGGCTTGTTTGTAGAAAAAATAGA
TGCCAATAGTTTGCTTAATTTTTATGCGGAATATATCAATGGCGTTCCTAGTGAATACACTTTTGCTAGAGGTCGTTTGC
ATGATGGCATGATCAATTCTGATGTGCATTTTAAAAAAGATTTTTTCACTCACATTCATAATAGTAAAGAGATTTTCAAG
CGTTTCATCAGCATTAAGACTTATGATATAGACAAAATTGTCAGCACTGCTCTTTCTTCAGTGTTGCATTTATTTTTAGA
ATTTGATGTGATTTTAAACATTGACAATATTCCTAAAGCAAAGGCTGAAAAAAGGATTGAAACTAAAAGAAAAAGAGCGA
ATAAGAGCATTAGAGTTGAAATTGATGAATTGAATGACATGATTAAAACAGACAGGGTTTTGATGCAGGAGATTTCTTTA
AACATTTTAGTTCATGCCAAAACTAAAACCGATTTAGATAGTGCTTGTATAGAAATTACCAACTTGCTTAAACAAAAAGG
TATTGTAAGCGCGCAAGAGAGTATTGGTATGTTGCCTATGTTTTTTAGCTTTTTCCCCAATCGTAATCGCTTGAATTTTA
GAAAGCGTTTGTTGTCTAGTCAAAATATTGCTTCTTTAATCATATTGGAAAAACAAAATTGTGGTTTTTCTAGAAATTCA
TGGGGTGATCGCCATTTAACTATTTTTAGAAACCAAGACAAATCGCCTTATTTATTCAATTTCCATGCTTATGAGGCAAA
AAGAAAAAATGATATGGTATCAGGGCATACCATCATCTTTGGTGGCACAGGGGCTGGTAAAACCACTTTAATTGAATTTT
TAATCACCAATTGTTTTAAGTATGAAGATTTAAGTATTTTAGCTTTAGACAGAAATAATGGCATGCGTGTGATGACTGAG
TTTTTAGATGGTCAATACAACGATAGCAATGATTTTTACATTAATCCGTTTTCTTTAAAAGATACAAGCGCTAATTGCAC
CTTTTTAGTGAGTTGGTTGGCATTCATGCTCAATATTGATGAAGATACTAAAGATGAAAAAGAAAAAAAGACTTTAAGCG
CTTTATCCAAAACGATCAGAGTTTGCTATAACAATATCACAAAACAAGGGGGTGCTATTTTTAAGTTAAGAGATTTTAAA
GACACGCTAGAAAGAGACTATTTAGATAGTAAAGACATTTTAGAAAAAGAAGGCTCAAAAGACTTATACAAGCATTCCAC
TGATTGTTTAGACTTTGCCAAAAAGCTTTCTGTTATTGATATGGATGCTCTTTCTAGCAATAAAAAAGATTTCAACTTGG
CAGTGCCATACCTATTCTACAAAGTCATGAATAGAGCTAAATTAGAAAACAAGCCTTTTTATATATTCATTGATGAAGCA
AAATCTGTTATTGAAAATCCCATCATGTTAGCTAAAATCAAAGACACACTCGCTCAAGCTAGAAAACTCAATGGAGTTTT
AACTTTAGCTTTTCAAGACATTAACCAACTTGATGGTGTAGAGGGAGCAAAGTCCATTATAGAAAACGCCGCTCAAGTCA
TACTCTATCCTACAAACAATTTTGAGAAATTAGAGAGCTATGGCATTTTATTAAGTCCTATTGAAAAATCATTTTTAAAC
AAAACGCCGTTAAACGCTAGGCAAGTATTAGTGAAAAACCTTCTCACTCAATCTAGCGTATTTTTAGAAATCAATTTAGA
AAAACTAAACAGCACCAGTAAAAATTTTTTAAGAGCGTATAGCTCTAGTGCGTCTAGCGTTTTTGAACTGGAAACGCTTA
AAAAGGATAATCCAAAGAATTATAAAGAAATTTACTTGACAAAGGAGTAG

Domains

Predicted by InterProScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (632-706)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (176-374)

• Helicase HerA, central domain (DUF87) (430-472)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comB4	Helicobacter pylori 26695	36.101	100	0.364