Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I653_RS00550 Genome accession   NC_020832
Coordinates   105511..106887 (+) Length   458 a.a.
NCBI ID   WP_015482702.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. BAB-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100511..111887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I653_RS00530 (I653_00415) ctsR 100864..101328 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  I653_RS00535 (I653_00420) mcsA 101342..101899 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  I653_RS00540 (I653_00425) mcsB 101899..102990 (+) 1092 WP_003235007.1 protein arginine kinase -
  I653_RS00545 (I653_00430) clpC 102987..105419 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  I653_RS00550 (I653_00435) radA 105511..106887 (+) 1377 WP_015482702.1 DNA repair protein RadA Machinery gene
  I653_RS00555 (I653_00440) disA 106891..107973 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  I653_RS00560 (I653_00445) yacL 108089..109189 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  I653_RS00565 (I653_00450) ispD 109204..109902 (+) 699 WP_015382584.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I653_RS00570 (I653_00455) ispF 109895..110371 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49426.92 Da        Isoelectric Point: 8.2393

>NTDB_id=57486 I653_RS00550 WP_015482702.1 105511..106887(+) (radA) [Bacillus subtilis subsp. subtilis str. BAB-1]
MAKTKSKFTCQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKKVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=57486 I653_RS00550 WP_015482702.1 105511..106887(+) (radA) [Bacillus subtilis subsp. subtilis str. BAB-1]
ATGGCTAAAACAAAATCGAAATTCACCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACACAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATCGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTGCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGGCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAGACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAAGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAGGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
TGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTTGTTGAAATTCAGGCGCTTATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAAAGTAGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

62.914

98.908

0.622
  radA Streptococcus pneumoniae D39

62.914

98.908

0.622
  radA Streptococcus pneumoniae R6

62.914

98.908

0.622
  radA Streptococcus pneumoniae TIGR4

62.914

98.908

0.622
  radA Streptococcus mitis NCTC 12261

62.914

98.908

0.622
  radA Streptococcus mitis SK321

62.693

98.908

0.62

Multiple sequence alignment