Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KM776_RS01790 Genome accession   NZ_CP076290
Coordinates   363602..364039 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Q12     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 358602..369039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM776_RS20910 - 359182..359343 (-) 162 Protein_306 hypothetical protein -
  KM776_RS01755 (KM776_01755) - 359460..360662 (+) 1203 WP_057080005.1 GTP-binding protein -
  KM776_RS01760 (KM776_01760) - 360727..361863 (+) 1137 WP_069013092.1 zinc-dependent alcohol dehydrogenase -
  KM776_RS01765 (KM776_01765) - 361878..362312 (+) 435 WP_057080003.1 RDD family protein -
  KM776_RS01770 (KM776_01770) - 362384..362704 (+) 321 WP_044053437.1 YckD family protein -
  KM776_RS01775 (KM776_01775) - 362808..362924 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  KM776_RS01780 (KM776_01780) - 362923..363141 (+) 219 Protein_312 family 1 glycosylhydrolase -
  KM776_RS01785 (KM776_01785) nin/comJ 363182..363580 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  KM776_RS01790 (KM776_01790) nucA/comI 363602..364039 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KM776_RS01795 (KM776_01795) hxlB 364398..364955 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  KM776_RS01800 (KM776_01800) hxlA 364952..365587 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  KM776_RS01805 (KM776_01805) - 365819..366181 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=574437 KM776_RS01790 WP_003156588.1 363602..364039(-) (nucA/comI) [Bacillus velezensis strain Q12]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=574437 KM776_RS01790 WP_003156588.1 363602..364039(-) (nucA/comI) [Bacillus velezensis strain Q12]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641