Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KM132_RS18210 Genome accession   NZ_CP076119
Coordinates   3717118..3718263 (+) Length   381 a.a.
NCBI ID   WP_022553933.1    Uniprot ID   -
Organism   Bacillus velezensis strain NZ4     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3712118..3723263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM132_RS18190 (KM132_18190) - 3712592..3713308 (-) 717 WP_007407631.1 Crp/Fnr family transcriptional regulator -
  KM132_RS18195 (KM132_18195) narK 3713406..3714596 (-) 1191 WP_154817753.1 nitrate transporter NarK -
  KM132_RS18200 (KM132_18200) argS 3714731..3716401 (-) 1671 WP_014419127.1 arginine--tRNA ligase -
  KM132_RS18205 (KM132_18205) - 3716398..3716826 (-) 429 WP_014419128.1 DUF1934 family protein -
  KM132_RS18210 (KM132_18210) rapF 3717118..3718263 (+) 1146 WP_022553933.1 tetratricopeptide repeat protein Regulator
  KM132_RS18215 (KM132_18215) - 3718247..3718366 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  KM132_RS18220 (KM132_18220) - 3718564..3718767 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  KM132_RS18225 (KM132_18225) - 3718781..3718984 (+) 204 WP_007407641.1 hypothetical protein -
  KM132_RS18230 (KM132_18230) - 3719019..3719195 (+) 177 Protein_3564 DUF4177 domain-containing protein -
  KM132_RS18235 (KM132_18235) - 3719390..3719671 (+) 282 WP_007407643.1 hypothetical protein -
  KM132_RS18240 (KM132_18240) - 3719715..3720227 (+) 513 WP_031304384.1 stage II sporulation protein M -
  KM132_RS18245 (KM132_18245) - 3720818..3721036 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  KM132_RS18250 (KM132_18250) speB 3721191..3722063 (-) 873 WP_007614448.1 agmatinase -
  KM132_RS18255 (KM132_18255) speE 3722122..3722952 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45555.57 Da        Isoelectric Point: 4.5797

>NTDB_id=572143 KM132_RS18210 WP_022553933.1 3717118..3718263(+) (rapF) [Bacillus velezensis strain NZ4]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=572143 KM132_RS18210 WP_022553933.1 3717118..3718263(+) (rapF) [Bacillus velezensis strain NZ4]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGAGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.215

100

0.782
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57