Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KM132_RS01835 Genome accession   NZ_CP076119
Coordinates   343339..343776 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain NZ4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 338339..348776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM132_RS01810 (KM132_01810) - 339360..340496 (+) 1137 WP_154817979.1 zinc-dependent alcohol dehydrogenase -
  KM132_RS01815 (KM132_01815) - 340511..340945 (+) 435 WP_021494203.1 RDD family protein -
  KM132_RS01820 (KM132_01820) - 341017..341340 (+) 324 WP_007409357.1 YckD family protein -
  KM132_RS01825 (KM132_01825) - 341444..342879 (+) 1436 Protein_322 family 1 glycosylhydrolase -
  KM132_RS01830 (KM132_01830) nin/comJ 342920..343318 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  KM132_RS01835 (KM132_01835) nucA/comI 343339..343776 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KM132_RS01840 (KM132_01840) hxlB 344107..344664 (-) 558 WP_109566681.1 6-phospho-3-hexuloisomerase -
  KM132_RS01845 (KM132_01845) hxlA 344661..345296 (-) 636 WP_109566682.1 3-hexulose-6-phosphate synthase -
  KM132_RS01850 (KM132_01850) - 345528..345890 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=572079 KM132_RS01835 WP_003156588.1 343339..343776(-) (nucA/comI) [Bacillus velezensis strain NZ4]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=572079 KM132_RS01835 WP_003156588.1 343339..343776(-) (nucA/comI) [Bacillus velezensis strain NZ4]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641