Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KMZ31_RS01850 Genome accession   NZ_CP076117
Coordinates   338992..339429 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain CQN-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 333992..344429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ31_RS01820 (KMZ31_01780) - 334846..336048 (+) 1203 WP_032872800.1 GTP-binding protein -
  KMZ31_RS01825 (KMZ31_01785) - 336113..337249 (+) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  KMZ31_RS01830 (KMZ31_01790) - 337264..337698 (+) 435 WP_021494203.1 RDD family protein -
  KMZ31_RS01835 (KMZ31_01795) - 337771..338094 (+) 324 WP_032872805.1 YckD family protein -
  KMZ31_RS01840 (KMZ31_01805) - 338314..338532 (+) 219 Protein_322 family 1 glycosylhydrolase -
  KMZ31_RS01845 (KMZ31_01810) nin/comJ 338573..338971 (-) 399 WP_032872809.1 competence protein ComJ Regulator
  KMZ31_RS01850 (KMZ31_01815) nucA/comI 338992..339429 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KMZ31_RS01855 (KMZ31_01820) hxlB 339786..340343 (-) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  KMZ31_RS01860 (KMZ31_01825) hxlA 340340..340975 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  KMZ31_RS01865 (KMZ31_01830) - 341207..341569 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=572003 KMZ31_RS01850 WP_003156588.1 338992..339429(-) (nucA/comI) [Bacillus amyloliquefaciens strain CQN-2]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=572003 KMZ31_RS01850 WP_003156588.1 338992..339429(-) (nucA/comI) [Bacillus amyloliquefaciens strain CQN-2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641