Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KLP38_RS01630 Genome accession   NZ_CP076060
Coordinates   345253..346737 (-) Length   494 a.a.
NCBI ID   WP_215529181.1    Uniprot ID   -
Organism   Cupriavidus sp. EM10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 340253..351737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLP38_RS01600 (KLP38_01600) - 340535..341188 (-) 654 WP_215529178.1 LON peptidase substrate-binding domain-containing protein -
  KLP38_RS01605 (KLP38_01605) rapZ 341246..342193 (-) 948 WP_215529179.1 RNase adapter RapZ -
  KLP38_RS01610 (KLP38_01610) - 342322..342657 (-) 336 WP_215529180.1 PsiF family protein -
  KLP38_RS01615 (KLP38_01615) hprK 342858..343829 (-) 972 WP_092603200.1 HPr(Ser) kinase/phosphatase -
  KLP38_RS01620 (KLP38_01620) ptsN 343895..344350 (-) 456 WP_066739687.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KLP38_RS01625 (KLP38_01625) hpf 344849..345202 (-) 354 WP_066739680.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KLP38_RS01630 (KLP38_01630) rpoN/rpoN1 345253..346737 (-) 1485 WP_215529181.1 RNA polymerase factor sigma-54 Machinery gene
  KLP38_RS01635 (KLP38_01635) lptB 346903..347703 (-) 801 WP_225934335.1 LPS export ABC transporter ATP-binding protein -
  KLP38_RS01640 (KLP38_01640) lptA 347727..348371 (-) 645 WP_215529182.1 lipopolysaccharide transport periplasmic protein LptA -
  KLP38_RS01645 (KLP38_01645) lptC 348436..349065 (-) 630 WP_215529183.1 LPS export ABC transporter periplasmic protein LptC -
  KLP38_RS01650 (KLP38_01650) - 349069..350051 (-) 983 Protein_334 SIS domain-containing protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 54986.39 Da        Isoelectric Point: 4.9230

>NTDB_id=571739 KLP38_RS01630 WP_215529181.1 345253..346737(-) (rpoN/rpoN1) [Cupriavidus sp. EM10]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
ANGDDRGEHAAAGGEEEGFGDSGGEDYGNDWSLDDFARRPQNDEDEKTPMQLRDADPTLREYLMEQLTPLKISTRDKGLA
IFLIESLDDDGYLSASLEEICTELPEELEFEVDEVQAILTLLQSFDPPGVGARNAAECLSLQLKRLKHPQRELALTIVNH
HLELLAVRDYTRLKKALQVDEIVLKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSGWIAQLNPDVMPRLRINDM
YAQILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTIS
RVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYR
EALKIPAVNLRKSL

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=571739 KLP38_RS01630 WP_215529181.1 345253..346737(-) (rpoN/rpoN1) [Cupriavidus sp. EM10]
ATGAAACCTTCGCTTCAGCTCCGCCTCTCCCAGCATCTGGCCCTGACGCCGCAACTGCAGCAGTCCATTCGCCTGCTGCA
GCTTTCCACGCTCGAACTGCAACAGGAGGTTGAGCAGGCACTGACGGAAAACCCGCTGCTCGAACGCGAGAATGACTGGA
TCGAAAGCCCTTTGCGCGTGGCGGCCGACGGCTCCGTCAACCTGCAGAGCGCCCCGGCGCCGGCTCCGGCCGAACCGCAG
GCCAATGGCGACGACCGTGGCGAGCATGCCGCCGCCGGCGGCGAGGAAGAAGGCTTCGGCGATTCCGGCGGCGAGGACTA
CGGCAACGACTGGAGCCTTGACGACTTCGCGCGTCGTCCGCAGAACGACGAGGACGAGAAAACGCCGATGCAGCTGCGCG
ACGCCGATCCCACGCTGCGTGAATACCTGATGGAACAGCTGACGCCGCTGAAGATTTCGACGCGCGACAAGGGCCTGGCC
ATCTTCCTGATCGAATCGCTCGACGACGACGGATACCTGAGTGCGTCACTCGAGGAAATCTGCACCGAACTGCCGGAAGA
ACTTGAATTCGAGGTAGACGAGGTGCAGGCCATTCTGACACTGCTGCAGAGCTTCGATCCGCCGGGCGTAGGCGCGCGCA
ATGCCGCCGAATGCCTGAGCCTGCAGCTGAAGCGCCTGAAGCATCCGCAACGCGAGCTGGCCTTGACCATCGTCAATCAT
CATCTCGAACTGCTGGCCGTCAGGGATTACACGCGTCTCAAGAAAGCGCTGCAGGTTGACGAAATCGTATTAAAGGCGGC
GCACGATCTGATCCGCTCGCTGGCACCGTATCCGGGGCATGCATACAGCCGCCCAGAAGCGGATTTCGTGGTGCCCGACG
TGTTCGTGCGCAAGAGTGGCAGCGGCTGGATTGCCCAGTTGAATCCGGACGTGATGCCTCGCCTGCGCATCAACGACATG
TACGCGCAAATTTTGCGCGGTGCAAAAGGAGAAGCCGGCGCCGCAGGACTGCAGCAGAAGCTGCAGGAAGCGCGGTGGCT
TATCAAGAACATCCAGCAAAGATTCGACACGATCCTGCGTGTGGCGCAGGCGATTGTCGAACGTCAGAAGAACTTTTTTT
CGCACGGCGAAATCGCAATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTCGGTTTACATGAGTCGACCATTTCC
CGGGTGACCACGAACAAATACATGGCCACGCCAATGGGTACTTTCGAGTTGAAGTACTTCTTTGGTAGCCACGTCTCCAC
GGAGACGGGCGGCGCGGCCTCATCCACGGCGATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGACCCCAGGAATC
CTCTTTCCGACAGCCGCATCGCCGAACTGCTGGGCGAACAGGGCTTTGTGGTCGCACGCCGCACGGTGGCCAAATACCGC
GAAGCGCTCAAGATCCCCGCAGTGAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-321)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.482

100

0.808