Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KB891_RS01650 Genome accession   NZ_CP076049
Coordinates   348796..350274 (-) Length   492 a.a.
NCBI ID   WP_017511025.1    Uniprot ID   -
Organism   Cupriavidus metallidurans strain YL2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343796..355274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KB891_RS01620 (KB891_01620) - 344169..344822 (-) 654 WP_011515184.1 LON peptidase substrate-binding domain-containing protein -
  KB891_RS01625 (KB891_01625) rapZ 344869..345759 (-) 891 WP_024569510.1 RNase adapter RapZ -
  KB891_RS01630 (KB891_01630) - 345874..346215 (-) 342 WP_017511023.1 PsiF family protein -
  KB891_RS01635 (KB891_01635) hprK 346421..347392 (-) 972 WP_008650955.1 HPr(Ser) kinase/phosphatase -
  KB891_RS01640 (KB891_01640) ptsN 347461..347916 (-) 456 WP_017511024.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KB891_RS01645 (KB891_01645) hpf 348392..348745 (-) 354 WP_008650960.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KB891_RS01650 (KB891_01650) rpoN/rpoN1 348796..350274 (-) 1479 WP_017511025.1 RNA polymerase factor sigma-54 Machinery gene
  KB891_RS01655 (KB891_01655) lptB 350398..351189 (-) 792 WP_011515190.1 LPS export ABC transporter ATP-binding protein -
  KB891_RS01660 (KB891_01660) lptA 351215..351859 (-) 645 WP_011515191.1 lipopolysaccharide transport periplasmic protein LptA -
  KB891_RS01665 (KB891_01665) lptC 351922..352548 (-) 627 WP_017511026.1 LPS export ABC transporter periplasmic protein LptC -
  KB891_RS01670 (KB891_01670) - 352552..353535 (-) 984 WP_024569509.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54833.08 Da        Isoelectric Point: 4.9624

>NTDB_id=571663 KB891_RS01650 WP_017511025.1 348796..350274(-) (rpoN/rpoN1) [Cupriavidus metallidurans strain YL2]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPASSEPQ
GNGDDRTNGANGEDDSYGDSASDDYGSDWSLDDFARRPQGDDDEKSPMQLREAEPTLREYLMEQLTPLKISTRDKGLAIF
LIESLDDDGYLGATLDEICSELPKELEFEVDEVQAILTLLQSFDPPGVGARNAAECLTLQLRRLQHPQRDLALSIVNHHL
ELLAVRDYTRLKKALQVDEIALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMYA
QILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQRNFFSHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEEPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKTL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=571663 KB891_RS01650 WP_017511025.1 348796..350274(-) (rpoN/rpoN1) [Cupriavidus metallidurans strain YL2]
ATGAAACCATCGCTTCAGCTTCGACTTTCCCAGCATCTGGCTCTGACTCCTCAACTGCAGCAGTCCATCCGGCTGCTGCA
GTTATCCACACTGGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCCGCGGACGGGTCGGTCAACTTGCAAAGCGCCCCGGCGCCAGCATCTTCCGAACCGCAA
GGTAACGGCGACGATCGCACGAACGGCGCCAATGGCGAAGACGACAGCTACGGCGACAGCGCGAGTGACGATTACGGCAG
CGACTGGAGCCTGGACGATTTCGCTCGCCGCCCTCAGGGCGACGATGACGAGAAGTCGCCGATGCAGCTTCGCGAAGCGG
AACCCACGCTGCGCGAATACCTGATGGAACAGCTCACGCCGCTGAAGATCTCCACGCGTGACAAGGGCCTGGCCATCTTC
CTGATCGAGTCGCTCGACGACGACGGCTACCTGGGCGCAACGCTCGACGAGATCTGTTCGGAACTGCCCAAGGAACTCGA
ATTCGAAGTCGATGAAGTGCAGGCGATACTGACCCTGCTGCAGAGCTTCGACCCGCCTGGCGTGGGTGCGCGCAACGCGG
CGGAATGCCTCACCCTGCAACTGCGCCGTCTGCAACATCCGCAGCGGGACCTTGCATTATCGATCGTCAACCACCACCTG
GAATTATTGGCCGTGCGTGACTACACGCGGCTCAAGAAAGCCCTGCAGGTTGACGAAATTGCACTTAAGGCCGCGCACGA
TCTGATCCGCTCGCTTGCACCGTATCCCGGACATGCCTACAGCCGTCCGGAAGCGGACTTCGTGGTACCGGATGTGTTCG
TGCGCAAGAGTGGCAGTAGCTGGATCGCGCAGCTCAATCCCGACGTGATGCCGCGTCTGCGCATCAACGACATGTACGCG
CAGATTTTGCGCGGTGCAAAAGGGGAAGCGGGTGCGGCCGGGCTGCAGCAGAAGCTCCAGGAGGCGCGGTGGCTCATCAA
GAACATTCAGCAGCGTTTCGACACGATCCTGCGTGTTGCGCAGGCAATTGTGGAACGTCAAAGGAACTTTTTCTCGCACG
GGGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTCTACACGAGTCGACCATTTCCCGGGTG
ACTACGAATAAATACATGGCCACGCCGATGGGTACTTTCGAGTTGAAGTACTTCTTTGGTAGCCACGTCTCCACGGAGAC
TGGCGGCGCGGCCTCATCCACGGCGATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGAACCCAGGAATCCCCTTT
CCGACAGCCGCATCGCCGAGTTGCTGGGCGAACAGGGCTTTGTGGTCGCACGCCGCACGGTGGCCAAGTACCGCGAAGCG
CTGAAGATCCCCGCAGTCAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.6

100

0.799