Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   MN178_RS29595 Genome accession   NZ_CP093356
Coordinates   6301200..6301796 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain E125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6296200..6306796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN178_RS29575 (MN178_29575) aroK 6297331..6297849 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MN178_RS29580 (MN178_29580) pilQ 6297861..6300005 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  MN178_RS29585 (MN178_29585) comL/pilP 6300059..6300583 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  MN178_RS29590 (MN178_29590) pilO 6300580..6301203 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  MN178_RS29595 (MN178_29595) pilN 6301200..6301796 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  MN178_RS29600 (MN178_29600) pilM 6301796..6302860 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  MN178_RS29605 (MN178_29605) - 6303045..6305516 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=571653 MN178_RS29595 WP_003095839.1 6301200..6301796(-) (pilN) [Pseudomonas aeruginosa strain E125]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=571653 MN178_RS29595 WP_003095839.1 6301200..6301796(-) (pilN) [Pseudomonas aeruginosa strain E125]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369