Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KH238_RS00065 Genome accession   NZ_CP076045
Coordinates   15283..15720 (-) Length   145 a.a.
NCBI ID   WP_032867344.1    Uniprot ID   -
Organism   Bacillus velezensis strain VCN56     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 10283..20720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH238_RS00040 (KH238_00040) - 11303..12439 (+) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -
  KH238_RS00045 (KH238_00045) - 12454..12888 (+) 435 WP_007409358.1 RDD family protein -
  KH238_RS00050 (KH238_00050) - 12960..13283 (+) 324 WP_007409357.1 YckD family protein -
  KH238_RS00055 (KH238_00055) - 13387..14823 (+) 1437 WP_032867342.1 family 1 glycosylhydrolase -
  KH238_RS00060 (KH238_00060) nin/comJ 14864..15262 (-) 399 WP_015239128.1 competence protein ComJ Regulator
  KH238_RS00065 (KH238_00065) nucA/comI 15283..15720 (-) 438 WP_032867344.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KH238_RS00070 (KH238_00070) hxlB 16078..16635 (-) 558 WP_032867345.1 6-phospho-3-hexuloisomerase -
  KH238_RS00075 (KH238_00075) hxlA 16632..17267 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  KH238_RS00080 (KH238_00080) - 17500..17862 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16094.41 Da        Isoelectric Point: 9.0073

>NTDB_id=571579 KH238_RS00065 WP_032867344.1 15283..15720(-) (nucA/comI) [Bacillus velezensis strain VCN56]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSHYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=571579 KH238_RS00065 WP_032867344.1 15283..15720(-) (nucA/comI) [Bacillus velezensis strain VCN56]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTCATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641