Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   MNY35_RS15140 Genome accession   NZ_CP093298
Coordinates   3165715..3168033 (+) Length   772 a.a.
NCBI ID   WP_062623556.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MN-13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3160715..3173033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY35_RS15120 (MNY35_15105) - 3162798..3163469 (+) 672 WP_012118444.1 DsbA family protein -
  MNY35_RS15125 (MNY35_15110) - 3163459..3163878 (+) 420 WP_015418211.1 disulfide oxidoreductase -
  MNY35_RS15130 (MNY35_15115) - 3163912..3164712 (-) 801 WP_040238889.1 zinc-dependent metalloprotease -
  MNY35_RS15135 (MNY35_15120) - 3164935..3165543 (+) 609 WP_007410017.1 trimeric intracellular cation channel family protein -
  MNY35_RS15140 (MNY35_15125) helD 3165715..3168033 (+) 2319 WP_062623556.1 RNA polymerase recycling motor HelD Machinery gene
  MNY35_RS15145 (MNY35_15130) - 3168208..3170016 (+) 1809 WP_007410019.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  MNY35_RS15150 (MNY35_15135) cysI 3170044..3171759 (+) 1716 WP_039253860.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -

Sequence

Protein


Download         Length: 772 a.a.        Molecular weight: 88145.09 Da        Isoelectric Point: 6.0350

>NTDB_id=571237 MNY35_RS15140 WP_062623556.1 3165715..3168033(+) (helD) [Bacillus amyloliquefaciens strain MN-13]
MAKRDKEWKEEQERINFVMKELEQKQTHLEASSGGLKQDIVGLRKTFWDDVTVNMDDAHEAAETFSSIKQQAEMLSERER
THRLQYRQLQKIQKLKTSPYFGRVDFTEKGESSSERIYIGLTSLMDENNEQFLIYDWRAPISSLYYRYSPGKARYEVPEE
TIEGDITLKRQFIIKNGTLKAMFNTDMTIGDEMLQEVLSGHSGQHMKNIVSTIQKEQNEIIRDEKSKYLIVQGAAGSGKT
SAALQRAAYLLYRGRGVLDARQIVLFSPNILFNSYVSAVLPELGEENMEQATFQEYIIRRLGRKFDCESPYEQLEYCLTE
QNQDALSVRLAAIAYKASSAFESMIDRYAGSLSGSGLMCKQISFRGDILISKERIEAYFYSLDQQLPIPNRAELTAEWLL
AEISALEKKERKKEWVLQESELLDKEDYAEVYQTLKKQAADDDEPAFDDHQREQRLLGAIIVGKAFKPIKQAVRRLGFLD
IKRMYLRLFSDWGADEKPDHWESIGALTRNAFAERSLPYEDAAPYLYLQDLLEGKRKNTQMKHVFIDEAQDYSPFQLTYI
QSIFPAAKMTVLGDVSQSIYAHTITGPSSLAGCFRNGAEKYVSLKRTYRSTRQIVEFTKALLADGNDIEPFNRNGEKPRV
LRTDTENARQEQLADTIQELRQKGWETVAVICKTAAEAKKVFQAVSAESGVRLINKENQPFQKGVCVIPVYLAKGIEFDA
VIAADASAVQYKSEHDRKLLYTACTRAMHHLTVLSPGEPSPFIKEVPAGLFV

Nucleotide


Download         Length: 2319 bp        

>NTDB_id=571237 MNY35_RS15140 WP_062623556.1 3165715..3168033(+) (helD) [Bacillus amyloliquefaciens strain MN-13]
ATGGCAAAACGTGATAAAGAGTGGAAAGAAGAGCAGGAAAGAATCAATTTTGTCATGAAGGAACTGGAACAGAAACAAAC
TCATCTTGAAGCCTCATCGGGCGGATTGAAGCAGGATATTGTCGGGCTGAGAAAAACGTTTTGGGATGATGTCACCGTGA
ATATGGATGACGCCCATGAAGCGGCTGAGACATTTTCAAGCATTAAACAGCAGGCAGAAATGCTTTCAGAACGTGAACGG
ACGCATCGGCTTCAATACCGGCAGCTTCAAAAAATCCAAAAGCTGAAAACCTCTCCTTACTTCGGCCGTGTCGATTTTAC
GGAAAAAGGGGAGTCCTCTTCCGAGCGGATTTATATCGGGCTGACTTCTTTGATGGATGAAAACAACGAGCAATTTCTCA
TTTATGATTGGCGCGCGCCCATCTCATCTCTTTACTATCGATATTCCCCGGGAAAAGCCCGCTATGAAGTTCCCGAAGAA
ACGATAGAAGGAGACATCACACTGAAGCGGCAGTTTATCATTAAAAACGGGACGCTTAAAGCGATGTTTAACACGGATAT
GACGATTGGTGACGAAATGCTGCAAGAGGTGCTGAGCGGCCATTCCGGACAGCATATGAAAAACATCGTGTCAACCATCC
AAAAAGAACAGAACGAAATCATCCGGGATGAAAAAAGCAAATATCTCATCGTTCAGGGAGCCGCCGGAAGCGGGAAAACA
TCCGCGGCGCTTCAGCGGGCCGCTTACTTGCTGTACCGCGGTCGCGGGGTGCTTGATGCCCGTCAGATTGTATTGTTTTC
CCCGAACATTTTGTTCAACAGTTATGTTTCCGCCGTATTGCCGGAATTGGGAGAAGAGAATATGGAGCAGGCCACCTTTC
AGGAATATATCATCCGCCGGCTCGGACGAAAGTTTGATTGTGAAAGCCCGTACGAGCAGCTTGAATATTGCCTGACTGAA
CAAAATCAGGACGCACTTTCTGTAAGGCTTGCAGCCATTGCTTACAAAGCGTCATCAGCTTTCGAGTCCATGATTGACCG
CTACGCCGGCTCGCTTTCCGGCTCGGGTCTGATGTGTAAACAGATCAGCTTCCGGGGAGACATCCTGATTTCAAAAGAAC
GGATTGAAGCCTATTTTTATTCGCTTGATCAGCAGCTGCCGATTCCGAACAGAGCGGAGCTGACAGCGGAATGGCTTCTC
GCGGAAATCAGCGCTCTCGAAAAAAAGGAGCGTAAGAAGGAATGGGTCTTGCAGGAGAGCGAGCTGCTTGATAAGGAAGA
CTATGCTGAGGTTTATCAAACACTCAAGAAGCAGGCGGCAGATGATGATGAACCTGCATTTGACGACCATCAGCGCGAAC
AGCGGCTGCTTGGCGCCATTATTGTCGGCAAAGCCTTTAAACCGATTAAGCAGGCCGTCAGACGGCTCGGCTTTCTCGAT
ATAAAACGGATGTATCTCCGGCTGTTTTCAGACTGGGGAGCTGATGAAAAGCCCGATCATTGGGAGAGCATCGGCGCTTT
GACCAGAAACGCATTTGCAGAACGGTCACTTCCGTATGAAGATGCCGCTCCGTATCTGTATTTGCAGGATCTGTTAGAAG
GAAAAAGAAAAAACACACAAATGAAGCACGTCTTCATTGATGAAGCGCAGGATTATTCTCCATTTCAGCTGACGTATATT
CAAAGCATCTTCCCAGCTGCAAAGATGACGGTGCTCGGTGATGTCAGCCAGTCGATTTATGCCCACACCATTACGGGCCC
TTCTTCCCTCGCCGGCTGCTTTCGCAATGGTGCAGAAAAGTATGTCAGCCTGAAGCGGACTTACAGATCGACGCGGCAGA
TTGTTGAATTTACGAAAGCGCTGCTGGCTGACGGTAATGACATTGAGCCATTTAACCGGAACGGGGAAAAACCGCGTGTT
TTGCGGACGGATACAGAAAATGCGCGGCAGGAACAATTGGCGGATACAATCCAGGAGCTCAGACAAAAAGGCTGGGAGAC
GGTCGCCGTCATCTGCAAAACGGCCGCGGAAGCCAAAAAAGTGTTTCAGGCGGTCAGCGCCGAGAGCGGTGTCCGCTTGA
TCAATAAAGAAAACCAGCCTTTTCAAAAAGGGGTATGCGTCATTCCCGTTTATTTGGCAAAAGGCATTGAGTTTGACGCC
GTCATTGCTGCTGACGCCTCTGCTGTCCAGTACAAAAGCGAACATGACCGTAAGCTGTTATATACGGCCTGTACACGGGC
GATGCATCATCTGACCGTATTGTCTCCGGGAGAACCCTCTCCTTTTATTAAAGAAGTGCCCGCCGGCTTATTCGTGTAA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(705-753)

AAA domain (AAA_19)

(217-581)

UvrD-like helicase C-terminal domain (UvrD_C)

(602-687)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

66.667

99.87

0.666