Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KLF48_RS13500 Genome accession   NZ_CP075979
Coordinates   3010975..3012336 (-) Length   453 a.a.
NCBI ID   WP_003479244.1    Uniprot ID   A0A127ELS8
Organism   Clostridium perfringens strain CPI 18-6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3005975..3017336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLF48_RS13480 (KLF48_13455) ispD 3007347..3008024 (-) 678 WP_003464566.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KLF48_RS13485 (KLF48_13460) - 3008038..3009144 (-) 1107 WP_060671145.1 PIN/TRAM domain-containing protein -
  KLF48_RS13490 (KLF48_13465) - 3009456..3009857 (+) 402 WP_003452221.1 hypothetical protein -
  KLF48_RS13495 (KLF48_13470) disA 3009891..3010955 (-) 1065 WP_003452233.1 DNA integrity scanning diadenylate cyclase DisA -
  KLF48_RS13500 (KLF48_13475) radA 3010975..3012336 (-) 1362 WP_003479244.1 DNA repair protein RadA Machinery gene
  KLF48_RS13505 (KLF48_13480) nagB 3012588..3013316 (-) 729 WP_003452236.1 glucosamine-6-phosphate deaminase -
  KLF48_RS13510 (KLF48_13485) - 3013313..3014035 (-) 723 WP_003452208.1 GntR family transcriptional regulator -
  KLF48_RS13515 (KLF48_13490) - 3014235..3014399 (+) 165 WP_003452205.1 hypothetical protein -
  KLF48_RS13520 (KLF48_13495) - 3014493..3014720 (+) 228 WP_003452255.1 glutaredoxin family protein -
  KLF48_RS13525 (KLF48_13500) - 3014843..3016291 (-) 1449 WP_003452227.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  KLF48_RS13530 (KLF48_13505) - 3016308..3016541 (-) 234 WP_003452243.1 glutaredoxin family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49820.54 Da        Isoelectric Point: 6.5314

>NTDB_id=571198 KLF48_RS13500 WP_003479244.1 3010975..3012336(-) (radA) [Clostridium perfringens strain CPI 18-6]
MAKVRSIYVCQNCGYETPKWMGKCPECNNWNTLVEEIRDTKSNQSSPKVERQIGELKKIKEIKSGEKERYDTGIGELNRV
LGGGLVKGSLTLISGDPGIGKSTLLLQTANNISKKYGKVLYVSGEESEEQIKIRGDRLKVDAEELYIVSETNLDVIEAYI
DKLEPAFIIIDSIQTIYRETVSSAPGSVSQVKECSNAVMRIAKGKNIPLFIVAHVTKQGDLAGPRVLEHMVDTVLSFEGE
RTEEFRILRTMKNRFGTTAEIGVFEMRGEGLMQVYDPSSMFLEDTSFNQEGSVVIGVMEGTRPILVEIQSLASETKAVMP
RRTSVGVENSRLSLILAVLEKKLRVPFYNTDVYVNVVGGLEIEGTTADLGIAISLVSSVKGKAASLEKLVVVGEVGLTGE
IRPISNCDRILNEAEKMGFLNAVVPYRSLEKLKGSKLNLIGVKTVREAIGKIF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=571198 KLF48_RS13500 WP_003479244.1 3010975..3012336(-) (radA) [Clostridium perfringens strain CPI 18-6]
GTGGCAAAGGTTAGAAGCATATATGTATGTCAAAATTGTGGATATGAAACACCTAAATGGATGGGTAAATGTCCAGAATG
TAATAATTGGAATACTTTAGTTGAAGAGATTAGAGATACTAAGAGTAATCAATCTTCTCCTAAGGTTGAAAGACAAATTG
GTGAGCTTAAAAAGATAAAAGAAATTAAATCAGGTGAAAAGGAAAGATATGATACTGGAATTGGTGAATTAAATAGAGTA
TTAGGTGGAGGGCTTGTTAAGGGGTCACTTACACTTATATCTGGTGACCCTGGAATAGGTAAATCTACACTTCTTTTACA
AACAGCCAATAATATTTCAAAAAAATATGGGAAAGTTTTATATGTTTCTGGGGAAGAATCAGAAGAACAAATAAAAATAA
GAGGAGACAGATTAAAAGTTGATGCAGAAGAACTTTATATAGTTTCCGAAACTAATCTTGATGTAATAGAAGCATATATA
GATAAACTTGAACCAGCTTTCATAATAATAGATTCAATACAAACAATTTATAGAGAAACAGTTTCTTCAGCACCAGGAAG
TGTTTCACAGGTTAAGGAATGCTCAAATGCTGTAATGAGAATTGCTAAAGGAAAAAATATTCCTTTATTTATAGTTGCTC
ACGTTACTAAGCAAGGGGATTTAGCAGGACCAAGAGTATTAGAACACATGGTGGATACTGTATTATCCTTTGAAGGGGAA
AGAACTGAAGAATTTAGAATATTAAGAACCATGAAAAATAGATTTGGTACTACGGCTGAAATAGGTGTTTTTGAAATGCG
TGGAGAGGGATTAATGCAGGTTTATGATCCATCTAGCATGTTTTTAGAGGACACTAGTTTTAATCAAGAAGGGTCAGTTG
TAATTGGAGTCATGGAAGGAACTAGACCTATTCTTGTAGAAATTCAATCATTAGCATCTGAAACAAAAGCAGTTATGCCT
AGAAGAACCTCTGTAGGAGTAGAAAATTCAAGGTTAAGTTTAATATTAGCTGTATTAGAAAAGAAATTAAGAGTTCCTTT
TTATAATACTGATGTTTATGTAAATGTAGTAGGAGGACTTGAAATAGAAGGTACTACAGCAGACTTAGGAATTGCTATTT
CACTTGTATCCTCTGTTAAAGGTAAGGCAGCTAGCTTAGAAAAATTAGTTGTTGTAGGCGAGGTTGGGTTAACAGGTGAA
ATAAGACCAATATCAAATTGTGACAGAATATTAAATGAAGCAGAAAAAATGGGATTTTTAAATGCAGTTGTTCCTTATAG
AAGTTTAGAAAAATTAAAGGGAAGTAAATTAAACCTTATAGGAGTAAAAACTGTAAGGGAAGCAATAGGAAAGATATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-426)

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127ELS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.501
  radA Streptococcus pneumoniae Rx1

47.577

100

0.477
  radA Streptococcus pneumoniae D39

47.577

100

0.477
  radA Streptococcus pneumoniae R6

47.577

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.577

100

0.477
  radA Streptococcus mitis SK321

47.357

100

0.475
  radA Streptococcus mitis NCTC 12261

47.357

100

0.475