Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KLF31_RS08440 Genome accession   NZ_CP075966
Coordinates   1926449..1927501 (-) Length   350 a.a.
NCBI ID   WP_011592647.1    Uniprot ID   Q0SS55
Organism   Clostridium perfringens strain 318/86     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1921449..1932501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLF31_RS08420 (KLF31_08400) sigE 1922043..1922750 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  KLF31_RS08425 (KLF31_08405) spoIIGA 1922764..1923570 (-) 807 WP_115650118.1 sigma-E processing peptidase SpoIIGA -
  KLF31_RS08430 (KLF31_08410) ftsZ 1923974..1925119 (-) 1146 WP_111916955.1 cell division protein FtsZ -
  KLF31_RS08435 (KLF31_08415) - 1925133..1926371 (-) 1239 WP_224374260.1 cell division FtsA domain-containing protein -
  KLF31_RS08440 (KLF31_08420) pilT 1926449..1927501 (-) 1053 WP_011592647.1 type IV pilus twitching motility protein PilT Machinery gene
  KLF31_RS08445 (KLF31_08425) sigK 1927682..1928383 (-) 702 WP_011590950.1 RNA polymerase sporulation sigma factor SigK -
  KLF31_RS08450 (KLF31_08430) - 1928446..1930113 (-) 1668 WP_224374261.1 penicillin-binding transpeptidase domain-containing protein -
  KLF31_RS08455 (KLF31_08435) udk 1930226..1930852 (-) 627 WP_011592650.1 uridine kinase -
  KLF31_RS08460 (KLF31_08440) - 1930852..1932081 (-) 1230 WP_111916959.1 U32 family peptidase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39020.72 Da        Isoelectric Point: 5.4507

>NTDB_id=571108 KLF31_RS08440 WP_011592647.1 1926449..1927501(-) (pilT) [Clostridium perfringens strain 318/86]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAKEILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETIDGERTASLEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=571108 KLF31_RS08440 WP_011592647.1 1926449..1927501(-) (pilT) [Clostridium perfringens strain 318/86]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTGCGCCCTGAAGATACTGAAGCGTATGCTAAAG
AGATATTACAGGATGCATATGAAAAATATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATCAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGAAAGGATACTTTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTGATTGGGGAAATGAGAGATTTGGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTGGATGTTTTTCCACCACATCAACAAGAGCAAATAAAAATACAGTTAGCTTCTGTTTTACAAACAATTATATCACAACA
ATTAGTGGAAACCATTGATGGAGAGAGAACTGCGTCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAACTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCTATCCAAACTGGAAGTAAATATGGAATGAGAACTATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAAATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0SS55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

98.286

0.491
  pilT Neisseria gonorrhoeae MS11

50

98.286

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

98.857

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain ERS1305867

49.123

97.714

0.48
  pilT Legionella pneumophila strain Lp02

49.123

97.714

0.48
  pilT Acinetobacter baylyi ADP1

50.301

94.857

0.477
  pilT Acinetobacter baumannii D1279779

49.405

96

0.474
  pilT Acinetobacter nosocomialis M2

49.405

96

0.474
  pilT Pseudomonas stutzeri DSM 10701

49.405

96

0.474
  pilT Acinetobacter baumannii strain A118

49.405

96

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

93.429

0.471
  pilT Vibrio cholerae strain A1552

50.459

93.429

0.471
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.296

97.429

0.383