Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KLF27_RS12980 Genome accession   NZ_CP075903
Coordinates   2864599..2865960 (-) Length   453 a.a.
NCBI ID   WP_003479244.1    Uniprot ID   A0A127ELS8
Organism   Clostridium perfringens strain CPS 2a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2859599..2870960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLF27_RS12960 (KLF27_12915) ispD 2860971..2861648 (-) 678 WP_003459324.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KLF27_RS12965 (KLF27_12920) - 2861662..2862768 (-) 1107 WP_003469613.1 PIN/TRAM domain-containing protein -
  KLF27_RS12970 (KLF27_12925) - 2863080..2863481 (+) 402 WP_003459340.1 hypothetical protein -
  KLF27_RS12975 (KLF27_12930) disA 2863515..2864579 (-) 1065 WP_003452233.1 DNA integrity scanning diadenylate cyclase DisA -
  KLF27_RS12980 (KLF27_12935) radA 2864599..2865960 (-) 1362 WP_003479244.1 DNA repair protein RadA Machinery gene
  KLF27_RS12985 (KLF27_12940) nagB 2866211..2866939 (-) 729 WP_003472813.1 glucosamine-6-phosphate deaminase -
  KLF27_RS12990 (KLF27_12945) - 2866936..2867658 (-) 723 WP_198603438.1 GntR family transcriptional regulator -
  KLF27_RS12995 (KLF27_12950) - 2867858..2868022 (+) 165 WP_003459343.1 hypothetical protein -
  KLF27_RS13000 (KLF27_12955) - 2868116..2868343 (+) 228 WP_003452255.1 glutaredoxin family protein -
  KLF27_RS13005 (KLF27_12960) - 2868466..2869914 (-) 1449 WP_011010907.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  KLF27_RS13010 (KLF27_12965) - 2869931..2870164 (-) 234 WP_003452243.1 glutaredoxin family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49820.54 Da        Isoelectric Point: 6.5314

>NTDB_id=570820 KLF27_RS12980 WP_003479244.1 2864599..2865960(-) (radA) [Clostridium perfringens strain CPS 2a]
MAKVRSIYVCQNCGYETPKWMGKCPECNNWNTLVEEIRDTKSNQSSPKVERQIGELKKIKEIKSGEKERYDTGIGELNRV
LGGGLVKGSLTLISGDPGIGKSTLLLQTANNISKKYGKVLYVSGEESEEQIKIRGDRLKVDAEELYIVSETNLDVIEAYI
DKLEPAFIIIDSIQTIYRETVSSAPGSVSQVKECSNAVMRIAKGKNIPLFIVAHVTKQGDLAGPRVLEHMVDTVLSFEGE
RTEEFRILRTMKNRFGTTAEIGVFEMRGEGLMQVYDPSSMFLEDTSFNQEGSVVIGVMEGTRPILVEIQSLASETKAVMP
RRTSVGVENSRLSLILAVLEKKLRVPFYNTDVYVNVVGGLEIEGTTADLGIAISLVSSVKGKAASLEKLVVVGEVGLTGE
IRPISNCDRILNEAEKMGFLNAVVPYRSLEKLKGSKLNLIGVKTVREAIGKIF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=570820 KLF27_RS12980 WP_003479244.1 2864599..2865960(-) (radA) [Clostridium perfringens strain CPS 2a]
GTGGCAAAGGTTAGAAGCATATATGTATGTCAAAATTGTGGATATGAAACACCTAAATGGATGGGTAAATGTCCAGAATG
TAATAATTGGAATACTTTAGTTGAAGAGATTAGAGATACTAAGAGTAATCAATCTTCTCCTAAGGTTGAAAGACAAATTG
GTGAGCTTAAAAAGATAAAAGAAATTAAATCAGGTGAAAAGGAAAGATATGATACTGGAATTGGTGAATTAAATAGAGTA
TTAGGTGGAGGGCTTGTTAAGGGGTCACTTACACTTATATCTGGTGATCCTGGGATAGGTAAATCTACACTTCTTTTACA
GACAGCCAATAATATTTCAAAAAAATATGGGAAAGTTTTATATGTTTCTGGGGAAGAATCAGAAGAACAAATAAAAATAA
GAGGAGACAGATTAAAAGTTGATGCAGAAGAACTTTATATAGTTTCCGAAACTAATCTTGATGTAATAGAAGCATATATA
GATAAACTTGAACCAGCTTTCATAATAATAGATTCAATACAAACAATTTATAGAGAAACAGTTTCTTCAGCACCAGGAAG
TGTTTCACAGGTTAAGGAATGCTCAAATGCTGTAATGAGAATTGCTAAAGGAAAAAATATTCCTTTATTTATAGTTGCTC
ACGTTACTAAGCAAGGGGATTTAGCAGGACCAAGAGTATTAGAACACATGGTGGATACAGTATTATCCTTTGAAGGGGAA
AGAACTGAAGAATTTAGAATATTAAGAACCATGAAAAATAGATTTGGTACTACGGCTGAAATAGGTGTTTTTGAAATGCG
TGGAGAAGGATTAATGCAGGTTTATGATCCATCTAGCATGTTTTTAGAGGACACTAGTTTTAATCAAGAAGGGTCAGTTG
TAATTGGAGTCATGGAAGGAACTAGACCTATTCTTGTAGAAATTCAATCATTAGCATCTGAAACAAAAGCAGTTATGCCT
AGAAGAACCTCTGTAGGAGTAGAAAATTCAAGGTTAAGTTTAATATTAGCTGTATTAGAAAAAAAATTAAGAGTTCCTTT
TTATAATACTGATGTTTATGTAAATGTAGTAGGAGGACTTGAAATAGAAGGTACTACAGCAGACTTAGGAATTGCTATTT
CACTTGTATCCTCTGTTAAAGGTAAGGCAGCTAGCTTAGAAAAATTAGTTGTTGTAGGCGAGGTTGGGTTAACAGGTGAA
ATAAGACCAATATCAAATTGTGACAGAATATTAAATGAAGCAGAAAAAATGGGATTTTTAAATGCAGTTGTTCCTTATAG
AAGTTTAGAAAAATTAAAGGGAAGTAAATTAAACCTTATAGGAGTAAAAACTGTAAGGGAAGCAATAGGAAAGATATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-426)

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127ELS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.501
  radA Streptococcus pneumoniae Rx1

47.577

100

0.477
  radA Streptococcus pneumoniae D39

47.577

100

0.477
  radA Streptococcus pneumoniae R6

47.577

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.577

100

0.477
  radA Streptococcus mitis SK321

47.357

100

0.475
  radA Streptococcus mitis NCTC 12261

47.357

100

0.475