Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MM431_RS20215 Genome accession   NZ_CP093068
Coordinates   4130684..4131124 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain BR1220     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4125684..4136124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM431_RS20195 (MM431_20195) aroP 4126682..4128055 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  MM431_RS20200 (MM431_20200) ampE 4128098..4128952 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MM431_RS20205 (MM431_20205) ampD 4128949..4129500 (-) 552 WP_061357681.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MM431_RS20210 (MM431_20210) nadC 4129588..4130481 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MM431_RS20215 (MM431_20215) pilA 4130684..4131124 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  MM431_RS20220 (MM431_20220) pilB 4131134..4132519 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  MM431_RS20225 (MM431_20225) hofC 4132509..4133716 (+) 1208 Protein_3994 protein transport protein HofC -
  MM431_RS20230 (MM431_20230) guaC 4133751..4134794 (-) 1044 WP_001217338.1 GMP reductase -
  MM431_RS20235 (MM431_20235) - 4134950..4134994 (-) 45 WP_120795372.1 protein YacM -
  MM431_RS20240 (MM431_20240) coaE 4135019..4135639 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=570566 MM431_RS20215 WP_000360904.1 4130684..4131124(+) (pilA) [Escherichia coli strain BR1220]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=570566 MM431_RS20215 WP_000360904.1 4130684..4131124(+) (pilA) [Escherichia coli strain BR1220]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363