Detailed information NTG0570508

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: MM431_RS01095
• Genome accession: NZ_CP093068
• Coordinates: 221793..222776 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain BR1220
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 216793..227776

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MM431_RS01080 (MM431_01080)	dppA	217936..219543 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
MM431_RS25865	-	219577..219687 (-)	111	Protein_215	hypothetical protein	-
MM431_RS01085 (MM431_01085)	dppB	219851..220870 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
MM431_RS01090 (MM431_01090)	dppC	220880..221782 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
MM431_RS01095 (MM431_01095)	amiE	221793..222776 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
MM431_RS01100 (MM431_01100)	dppF	222773..223777 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
MM431_RS01105 (MM431_01105)	yhjV	223807..225078 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
MM431_RS01110 (MM431_01110)	ldrD	225554..225661 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
MM431_RS01115 (MM431_01115)	bcsG	225748..227427 (-)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
MM431_RS01120 (MM431_01120)	bcsF	227424..227615 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=570508 MM431_RS01095 WP_001196486.1 221793..222776(+) (amiE) [Escherichia coli strain BR1220]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=570508 MM431_RS01095 WP_001196486.1 221793..222776(+) (amiE) [Escherichia coli strain BR1220]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404