Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MM522_RS10480 Genome accession   NZ_CP093067
Coordinates   2050924..2051334 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain ATC-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2045924..2056334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM522_RS10450 (MM522_10450) - 2046011..2046151 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MM522_RS10455 (MM522_10455) yqeF 2046457..2047188 (-) 732 WP_101172379.1 SGNH/GDSL hydrolase family protein -
  MM522_RS10460 (MM522_10460) cwlH 2047440..2048192 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MM522_RS10465 (MM522_10465) yqeD 2048379..2049005 (+) 627 WP_101172378.1 TVP38/TMEM64 family protein -
  MM522_RS10470 (MM522_10470) gnd 2049024..2049917 (-) 894 WP_101172377.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MM522_RS10475 (MM522_10475) yqeB 2050169..2050891 (+) 723 WP_010886572.1 hypothetical protein -
  MM522_RS10480 (MM522_10480) nucA/comI 2050924..2051334 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MM522_RS10485 (MM522_10485) - 2051530..2051877 (+) 348 Protein_2061 sigma-70 family RNA polymerase sigma factor -
  MM522_RS10490 (MM522_10490) spoIVCA 2051908..2053368 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MM522_RS10495 (MM522_10495) - 2053326..2053504 (-) 179 Protein_2063 hypothetical protein -
  MM522_RS10500 (MM522_10500) arsC 2053859..2054278 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MM522_RS10505 (MM522_10505) acr3 2054290..2055330 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  MM522_RS10510 (MM522_10510) yqcK 2055353..2055793 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MM522_RS10515 (MM522_10515) arsR 2055852..2056169 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=570432 MM522_RS10480 WP_009967785.1 2050924..2051334(-) (nucA/comI) [Bacillus subtilis strain ATC-3]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=570432 MM522_RS10480 WP_009967785.1 2050924..2051334(-) (nucA/comI) [Bacillus subtilis strain ATC-3]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529