Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CPZ02_RS00525 Genome accession   NZ_AP017610
Coordinates   112576..113016 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 20Ec-P-124     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 107576..118016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPZ02_RS00505 (MRY16002_c0940) coaE 108066..108686 (-) 621 WP_001269512.1 dephospho-CoA kinase -
  CPZ02_RS29135 - 108711..108755 (+) 45 WP_120795372.1 protein YacM -
  CPZ02_RS00510 (MRY16002_c0950) guaC 108911..109954 (+) 1044 WP_001217338.1 GMP reductase -
  CPZ02_RS00515 (MRY16002_c0960) hofC 109989..111191 (-) 1203 WP_000157293.1 protein transport protein HofC -
  CPZ02_RS00520 (MRY16002_c0970) pilB 111181..112566 (-) 1386 WP_001025165.1 type II secretion system protein GspE Machinery gene
  CPZ02_RS00525 (MRY16002_c0980) pilA 112576..113016 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  CPZ02_RS00535 (MRY16002_c0990) nadC 113219..114112 (-) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CPZ02_RS00540 (MRY16002_c1000) ampD 114200..114751 (+) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CPZ02_RS00545 (MRY16002_c1010) ampE 114748..115602 (+) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  CPZ02_RS00550 (MRY16002_c1020) aroP 115645..117015 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=57043 CPZ02_RS00525 WP_000360904.1 112576..113016(-) (pilA) [Escherichia coli strain 20Ec-P-124]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=57043 CPZ02_RS00525 WP_000360904.1 112576..113016(-) (pilA) [Escherichia coli strain 20Ec-P-124]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment