Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KKP64_RS00185 Genome accession   NZ_CP075604
Coordinates   45394..46764 (+) Length   456 a.a.
NCBI ID   WP_216788118.1    Uniprot ID   -
Organism   Enterococcus faecalis strain 1207/14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 40394..51764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKP64_RS00160 (KKP64_00160) - 40884..41351 (-) 468 WP_002359228.1 VOC family protein -
  KKP64_RS00165 (KKP64_00165) - 41416..42363 (+) 948 WP_002404711.1 YafY family protein -
  KKP64_RS00170 (KKP64_00170) - 42455..43708 (-) 1254 WP_002404712.1 glutamate-5-semialdehyde dehydrogenase -
  KKP64_RS00175 (KKP64_00175) proB 43695..44510 (-) 816 WP_002389550.1 glutamate 5-kinase -
  KKP64_RS00180 (KKP64_00180) - 44830..45315 (+) 486 WP_002359232.1 dUTP diphosphatase -
  KKP64_RS00185 (KKP64_00185) radA 45394..46764 (+) 1371 WP_216788118.1 DNA repair protein RadA Machinery gene
  KKP64_RS00190 (KKP64_00190) - 46868..48013 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  KKP64_RS00195 (KKP64_00195) ispF 48032..48505 (+) 474 WP_002359233.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  KKP64_RS00200 (KKP64_00200) gltX 48558..50015 (+) 1458 WP_002381335.1 glutamate--tRNA ligase -
  KKP64_RS00205 (KKP64_00205) epsC 50285..50824 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49785.33 Da        Isoelectric Point: 7.3489

>NTDB_id=569835 KKP64_RS00185 WP_216788118.1 45394..46764(+) (radA) [Enterococcus faecalis strain 1207/14]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRVERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=569835 KKP64_RS00185 WP_216788118.1 45394..46764(+) (radA) [Enterococcus faecalis strain 1207/14]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCGCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTACGAGTGGAGCGTCTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGGGCCACCGGAT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCAGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

75.165

99.781

0.75
  radA Streptococcus pneumoniae D39

75.165

99.781

0.75
  radA Streptococcus pneumoniae R6

75.165

99.781

0.75
  radA Streptococcus pneumoniae TIGR4

75.165

99.781

0.75
  radA Streptococcus mitis NCTC 12261

75.165

99.781

0.75
  radA Streptococcus mitis SK321

75.165

99.781

0.75
  radA Bacillus subtilis subsp. subtilis str. 168

66.075

98.904

0.654