Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KHO27_RS25685 Genome accession   NZ_CP075595
Coordinates   5668353..5669720 (+) Length   455 a.a.
NCBI ID   WP_046857208.1    Uniprot ID   A0A2V4HF15
Organism   Pseudomonas sp. RC3H12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5663353..5674720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHO27_RS25655 (KHO27_25655) cydB 5663549..5664556 (+) 1008 WP_102684552.1 cytochrome d ubiquinol oxidase subunit II -
  KHO27_RS25660 (KHO27_25660) - 5664569..5664709 (+) 141 WP_102684551.1 DUF2474 domain-containing protein -
  KHO27_RS25665 (KHO27_25665) - 5664773..5665897 (-) 1125 WP_214500969.1 methyltransferase -
  KHO27_RS25670 (KHO27_25670) - 5665996..5666700 (-) 705 WP_214500970.1 autoinducer binding domain-containing protein -
  KHO27_RS25675 (KHO27_25675) - 5666916..5667692 (+) 777 WP_110704089.1 ferredoxin--NADP reductase -
  KHO27_RS25680 (KHO27_25680) mscL 5667761..5668174 (-) 414 WP_046857209.1 large-conductance mechanosensitive channel protein MscL -
  KHO27_RS25685 (KHO27_25685) radA 5668353..5669720 (+) 1368 WP_046857208.1 DNA repair protein RadA Machinery gene
  KHO27_RS25690 (KHO27_25690) - 5669793..5670296 (+) 504 WP_110704091.1 RNA polymerase sigma factor -
  KHO27_RS25695 (KHO27_25695) - 5670365..5671357 (+) 993 WP_214500971.1 FecR domain-containing protein -
  KHO27_RS25700 (KHO27_25700) - 5671554..5674223 (+) 2670 WP_214502019.1 TonB-dependent receptor -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48770.18 Da        Isoelectric Point: 6.8985

>NTDB_id=569815 KHO27_RS25685 WP_046857208.1 5668353..5669720(+) (radA) [Pseudomonas sp. RC3H12]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVESMIESGGAAAPSGRTGWTGQQAQIKTLAEVSVEEIPRFTTSSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVQMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETIIA
TARQEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=569815 KHO27_RS25685 WP_046857208.1 5668353..5669720(+) (radA) [Pseudomonas sp. RC3H12]
ATGGCCAAGGCCAAGCGTTTGTATGGCTGCACCGAGTGCGGCGCGACCTTCCCCAAGTGGGCCGGGCAGTGTGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAGAGCATGATCGAAAGCGGCGGCGCCGCGGCGCCGAGCGGGCGCACCGGCTGGACCG
GGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTGAGCGTCGAGGAGATCCCGCGCTTCACCACCAGCAGCACCGAGCTC
GACCGCGTCCTGGGCGGGGGCCTGGTGGATGGGTCGGTGGTGCTGATCGGGGGCGATCCGGGTATCGGCAAATCGACCAT
CCTCCTGCAGACCCTGTGCAACATCGCCGTGCAGATGCCAGCGCTGTATGTCACCGGCGAGGAGTCGCAGCAGCAGGTGG
CCATGCGTTCCCGGCGTCTGGGCCTGCCCCAGGACCAGCTCAAGGTCATGACCGAAACCTGCATCGAAACCATCATCGCC
ACCGCGCGCCAGGAAAAGCCGCGGGTGATGGTGATCGACTCGATCCAGACCATCTTCACCGAACAACTGCAGTCGGCACC
GGGTGGCGTGGCCCAGGTGCGCGAGAGTACGGCATTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCCTGG
TCGGCCACGTCACCAAGGAGGGCTCGTTGGCCGGTCCACGGGTGCTCGAGCACATGGTCGATACCGTACTGTATTTCGAG
GGCGAGTCCGATGGCCGCCTGCGCCTGCTGCGCGCAGTGAAAAACCGCTTTGGCGCGGTCAACGAACTGGGCGTGTTCGG
CATGACCGACCGCGGCCTCAAGGAAGTTTCCAATCCGTCGGCGATCTTCCTCAACCGCACCCAGGAAGAGGTGCCCGGCA
GCGTGGTCATGGCTACCTGGGAGGGCACCCGGCCGATGCTGGTGGAAGTCCAGGCGCTGGTCGACGACAGCCACCTGGCC
AACCCACGGCGGGTAACCCTGGGCCTGGACCAGAATCGCCTGGCCATGCTGCTGGCGGTGCTGCACCGGCACGGTGGCAT
CCCCACCCACGACCAGGACGTGTTCCTCAACGTCGTCGGTGGCGTGAAGGTGCTCGAGACCGCCTCCGACCTCGCGTTGC
TGGCGGCGGTGATGTCCAGCCTGCGCAACCGGCCGTTGGCCCATGGCCTGCTGGTGTTCGGCGAGATCGGCCTGTCGGGC
GAGGTGCGTCCGGTGCCCAGTGGCCAGGAGCGTCTGAAGGAAGCGGCAAAACATGGCTTCAAGCGTGCCATCGTGCCCAA
GGGCAACGCACCAAAAGAGGCTCCCGCCGGGTTGCAGGTGATCGCCGTGACCCGTCTGGAACAAGCGCTGGATGCACTGT
TCGAGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(354-429)

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V4HF15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.488
  radA Streptococcus pneumoniae Rx1

46.187

100

0.466
  radA Streptococcus pneumoniae D39

46.187

100

0.466
  radA Streptococcus pneumoniae TIGR4

46.187

100

0.466
  radA Streptococcus pneumoniae R6

46.187

100

0.466
  radA Streptococcus mitis NCTC 12261

45.969

100

0.464
  radA Streptococcus mitis SK321

46.154

100

0.462