Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SAI4T8_RS07550 Genome accession   NC_020533
Coordinates   1558672..1559646 (-) Length   324 a.a.
NCBI ID   WP_041174216.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus ST228     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1553672..1564646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAI4T8_RS07510 (SAI4T8_1011170) gcvT 1554173..1555264 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SAI4T8_RS07515 (SAI4T8_1011180) - 1555423..1555947 (-) 525 WP_001015120.1 shikimate kinase -
  SAI4T8_RS07520 - 1555937..1556083 (-) 147 WP_001792109.1 hypothetical protein -
  SAI4T8_RS07525 (SAI4T8_1011190) comGF 1556180..1556677 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAI4T8_RS07530 comGE 1556595..1556894 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  SAI4T8_RS07535 comGD 1556881..1557327 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAI4T8_RS07540 comGC 1557305..1557616 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAI4T8_RS07545 (SAI4T8_1011200) comGB 1557630..1558700 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAI4T8_RS07550 (SAI4T8_1011210) comGA 1558672..1559646 (-) 975 WP_041174216.1 competence type IV pilus ATPase ComGA Machinery gene
  SAI4T8_RS07555 (SAI4T8_1011220) - 1559698..1560321 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAI4T8_RS07560 - 1560318..1560647 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAI4T8_RS07565 (SAI4T8_1011230) - 1560647..1561633 (-) 987 WP_000161309.1 ROK family glucokinase -
  SAI4T8_RS07570 - 1561630..1561833 (-) 204 WP_000087561.1 YqgQ family protein -
  SAI4T8_RS07575 (SAI4T8_1011240) - 1561814..1563277 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  SAI4T8_RS07580 (SAI4T8_1011250) - 1563289..1563828 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  SAI4T8_RS07585 rpmG 1564037..1564186 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36924.98 Da        Isoelectric Point: 8.8516

>NTDB_id=56961 SAI4T8_RS07550 WP_041174216.1 1558672..1559646(-) (comGA) [Staphylococcus aureus subsp. aureus ST228]
MKILFQEIINKAIEMKANDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=56961 SAI4T8_RS07550 WP_041174216.1 1558672..1559646(-) (comGA) [Staphylococcus aureus subsp. aureus ST228]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAATGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment