Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KHS93_RS17955 Genome accession   NZ_CP075547
Coordinates   3633401..3634546 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus amyloliquefaciens strain SN16-1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3628401..3639546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHS93_RS17935 (KHS93_17935) - 3628876..3629592 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  KHS93_RS17940 (KHS93_17940) narK 3629690..3630880 (-) 1191 WP_044052814.1 nitrate transporter NarK -
  KHS93_RS17945 (KHS93_17945) argS 3631014..3632684 (-) 1671 WP_161503338.1 arginine--tRNA ligase -
  KHS93_RS17950 (KHS93_17950) - 3632681..3633109 (-) 429 WP_003151069.1 DUF1934 family protein -
  KHS93_RS17955 (KHS93_17955) rapF 3633401..3634546 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  KHS93_RS17960 (KHS93_17960) - 3634530..3634649 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  KHS93_RS17965 (KHS93_17965) - 3634848..3635051 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  KHS93_RS17970 (KHS93_17970) - 3635065..3635268 (+) 204 WP_003151062.1 hypothetical protein -
  KHS93_RS19840 - 3635303..3635488 (+) 186 Protein_3507 DUF4177 domain-containing protein -
  KHS93_RS17975 (KHS93_17975) - 3635676..3635957 (+) 282 WP_014306021.1 hypothetical protein -
  KHS93_RS17980 (KHS93_17980) - 3636001..3636513 (+) 513 WP_003151059.1 stage II sporulation protein M -
  KHS93_RS17985 (KHS93_17985) - 3637103..3637321 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  KHS93_RS17990 (KHS93_17990) speB 3637491..3638363 (-) 873 WP_003151054.1 agmatinase -
  KHS93_RS17995 (KHS93_17995) speE 3638423..3639253 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=569490 KHS93_RS17955 WP_003151067.1 3633401..3634546(+) (rapF) [Bacillus amyloliquefaciens strain SN16-1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=569490 KHS93_RS17955 WP_003151067.1 3633401..3634546(+) (rapF) [Bacillus amyloliquefaciens strain SN16-1]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTATCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53