Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KHS93_RS08610 Genome accession   NZ_CP075547
Coordinates   1778497..1778931 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SN16-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1773497..1783931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHS93_RS08605 (KHS93_08605) - 1776997..1778208 (-) 1212 WP_069473684.1 cytochrome P450 -
  KHS93_RS08610 (KHS93_08610) nucA/comI 1778497..1778931 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KHS93_RS08615 (KHS93_08615) - 1778991..1779758 (+) 768 WP_025851167.1 YoaK family protein -
  KHS93_RS08620 (KHS93_08620) - 1779779..1780141 (-) 363 WP_161503447.1 hypothetical protein -
  KHS93_RS08625 (KHS93_08625) - 1780333..1781661 (-) 1329 WP_161503446.1 S8 family peptidase -
  KHS93_RS08630 (KHS93_08630) - 1781841..1782074 (+) 234 WP_046341482.1 hypothetical protein -
  KHS93_RS08635 (KHS93_08635) - 1782330..1783037 (+) 708 WP_046341256.1 poly-gamma-glutamate hydrolase family protein -
  KHS93_RS08640 (KHS93_08640) - 1783097..1783549 (+) 453 WP_015388205.1 OsmC family protein -
  KHS93_RS08645 (KHS93_08645) - 1783563..1783916 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=569455 KHS93_RS08610 WP_003154085.1 1778497..1778931(+) (nucA/comI) [Bacillus amyloliquefaciens strain SN16-1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=569455 KHS93_RS08610 WP_003154085.1 1778497..1778931(+) (nucA/comI) [Bacillus amyloliquefaciens strain SN16-1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486