Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LJF86_RS10740 Genome accession   NZ_CP093011
Coordinates   2173390..2173830 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 131     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168390..2178830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJF86_RS10720 (LJF86_10720) aroP 2169390..2170760 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  LJF86_RS10725 (LJF86_10725) ampE 2170803..2171657 (-) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  LJF86_RS10730 (LJF86_10730) ampD 2171654..2172205 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LJF86_RS10735 (LJF86_10735) nadC 2172293..2173186 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LJF86_RS10740 (LJF86_10740) pilA 2173390..2173830 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  LJF86_RS10745 (LJF86_10745) pilB 2173840..2175225 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  LJF86_RS10750 (LJF86_10750) hofC 2175215..2176417 (+) 1203 WP_000157289.1 protein transport protein HofC -
  LJF86_RS10755 (LJF86_10755) guaC 2176452..2177495 (-) 1044 WP_001217320.1 GMP reductase -
  LJF86_RS10760 (LJF86_10760) - 2177651..2177695 (-) 45 WP_120795372.1 protein YacM -
  LJF86_RS10765 (LJF86_10765) coaE 2177720..2178340 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=569063 LJF86_RS10740 WP_000360914.1 2173390..2173830(+) (pilA) [Escherichia coli strain 131]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=569063 LJF86_RS10740 WP_000360914.1 2173390..2173830(+) (pilA) [Escherichia coli strain 131]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363