Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KIL00_RS01985 Genome accession   NZ_CP075344
Coordinates   372080..372529 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. subtilis strain A1 - Midalam     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 367080..377529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL00_RS01950 (KIL00_01950) yckA 367230..367910 (-) 681 WP_014478791.1 amino acid ABC transporter permease -
  KIL00_RS01955 (KIL00_01955) yckB 367920..368783 (-) 864 WP_014478792.1 transporter substrate-binding domain-containing protein -
  KIL00_RS01960 (KIL00_01960) - 368945..369142 (+) 198 WP_121509395.1 hypothetical protein -
  KIL00_RS01965 (KIL00_01965) yckC 369162..369617 (+) 456 WP_014478793.1 RDD family protein -
  KIL00_RS01970 (KIL00_01970) yckD 369699..370031 (+) 333 WP_014478794.1 YckD family protein -
  KIL00_RS01975 (KIL00_01975) bglC 370185..371618 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  KIL00_RS01980 (KIL00_01980) nin/comJ 371655..372053 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  KIL00_RS01985 (KIL00_01985) nucA/comI 372080..372529 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  KIL00_RS01990 (KIL00_01990) tlpC 372697..374418 (-) 1722 Protein_348 methyl-accepting chemotaxis protein TlpC -
  KIL00_RS01995 (KIL00_01995) hxlB 374529..375086 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  KIL00_RS02000 (KIL00_02000) hxlA 375092..375724 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KIL00_RS02005 (KIL00_02005) hxlR 375954..376316 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=568884 KIL00_RS01985 WP_014475770.1 372080..372529(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=568884 KIL00_RS01985 WP_014475770.1 372080..372529(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987