Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KIP84_RS16060 Genome accession   NZ_CP075323
Coordinates   3339194..3339994 (-) Length   266 a.a.
NCBI ID   WP_199977444.1    Uniprot ID   -
Organism   Acinetobacter sp. BHS4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3334194..3344994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIP84_RS16040 (KIP84_16045) - 3335259..3335957 (-) 699 WP_170247645.1 tetratricopeptide repeat protein -
  KIP84_RS16045 (KIP84_16050) hisS 3335986..3337278 (-) 1293 WP_213688903.1 histidine--tRNA ligase -
  KIP84_RS16050 (KIP84_16055) ispG 3337275..3338390 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KIP84_RS16055 (KIP84_16060) - 3338412..3339203 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  KIP84_RS16060 (KIP84_16065) pilF 3339194..3339994 (-) 801 WP_199977444.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KIP84_RS16065 (KIP84_16070) rlmN 3340009..3341244 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KIP84_RS16070 (KIP84_16075) ndk 3341368..3341799 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  KIP84_RS16075 (KIP84_16080) iscX 3341913..3342113 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  KIP84_RS16080 (KIP84_16085) - 3342213..3342764 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  KIP84_RS16085 (KIP84_16090) - 3342789..3344078 (-) 1290 WP_213689861.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30086.32 Da        Isoelectric Point: 9.0417

>NTDB_id=568779 KIP84_RS16060 WP_199977444.1 3339194..3339994(-) (pilF) [Acinetobacter sp. BHS4]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=568779 KIP84_RS16060 WP_199977444.1 3339194..3339994(-) (pilF) [Acinetobacter sp. BHS4]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.361

100

0.944
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744