Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI615_RS18065 Genome accession   NZ_CP075186
Coordinates   3814697..3816412 (+) Length   571 a.a.
NCBI ID   WP_226471542.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain G6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3809697..3821412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI615_RS18045 (KI615_17990) - 3810506..3811117 (+) 612 WP_226417028.1 lytic transglycosylase domain-containing protein -
  KI615_RS18050 (KI615_17995) ffh 3811118..3812476 (-) 1359 WP_226471539.1 signal recognition particle protein -
  KI615_RS18055 (KI615_18000) - 3812506..3813345 (+) 840 WP_226471540.1 inner membrane protein YpjD -
  KI615_RS18060 (KI615_18005) - 3813349..3814620 (+) 1272 WP_226471541.1 HlyC/CorC family transporter -
  KI615_RS18065 (KI615_18010) pilB 3814697..3816412 (+) 1716 WP_226471542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI615_RS18070 (KI615_18015) pilC 3816427..3817653 (+) 1227 WP_226417033.1 type II secretion system F family protein Machinery gene
  KI615_RS18075 (KI615_18020) pilD 3817653..3818501 (+) 849 WP_226417034.1 prepilin peptidase Machinery gene
  KI615_RS18080 (KI615_18025) - 3818572..3818835 (+) 264 WP_226417035.1 FmdB family zinc ribbon protein -
  KI615_RS18085 (KI615_18030) - 3818835..3819449 (+) 615 WP_226419944.1 DUF502 domain-containing protein -
  KI615_RS18090 (KI615_18035) aspS 3819467..3821269 (+) 1803 WP_226417036.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61824.20 Da        Isoelectric Point: 6.0483

>NTDB_id=568463 KI615_RS18065 WP_226471542.1 3814697..3816412(+) (pilB) [Dechloromonas denitrificans strain G6]
MAATPQNPPLSGLARALVQAGQLKEAEAEQLLSQANSAKTSLIEQIIASQKASALDIARFVSDTFGYPLLDLAAFDEAHI
PANAIDRKLISTHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVAKYAESAADTLKNLGAD
DINLDFLDEESVAKADEAAGQDIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREIATPPLAIKEKV
ASRIKVISRLNIAEKRVPQDGRMRLVLSKTRAIDFRVSTLPTLQGEKIVLRILDPTSATLGIEALGYEPEQKVALLDAVN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSIQLITAQRLARRLCNCKKPMTVPNQALLDAG
YKEADLDGSWTLYGPGGCDRCKGSGFKGRVGIYQVMPISEAMQRLIMSGANSLDLARQAKAEGVKDLRESGLLKVKQGVT
SLDEVLSTTNV

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=568463 KI615_RS18065 WP_226471542.1 3814697..3816412(+) (pilB) [Dechloromonas denitrificans strain G6]
ATGGCAGCAACCCCGCAAAACCCTCCTCTTAGCGGCCTGGCGCGTGCCCTTGTCCAGGCCGGGCAACTCAAGGAGGCAGA
GGCCGAACAGTTGCTGTCCCAAGCCAACAGCGCCAAAACATCGCTGATCGAGCAGATCATCGCCAGCCAGAAAGCATCGG
CGCTTGATATTGCACGTTTTGTCTCTGACACCTTCGGTTATCCGCTGCTCGATCTCGCCGCTTTCGATGAAGCGCACATT
CCGGCAAATGCAATCGACCGCAAGCTGATTTCAACGCACAAGGTCATTCCACTCAACAAACGCGGCAACCGGCTGTCGGT
GGCCATTGCCGACCCGACCAATCTGCGGGCGCTGGACGAGATCCGCTTCCAGACCGGCCTTGCCGTCGACCCAATTGTCG
TCGAGCATCCGAAACTGGCCCCGCTGGTTGCCAAGTACGCGGAATCAGCTGCCGACACGCTGAAGAATCTGGGTGCCGAC
GACATCAATCTGGACTTCCTTGACGAAGAGTCAGTGGCAAAGGCAGATGAAGCTGCCGGCCAGGACATCGATGATGCGCC
AGTAGTCAAATTCATTCAGAAAATGCTCCTGGATGCGATCAACGATGGCGCATCGGACATCCATTTCGAACCCTACGAAA
AGTTTTACCGTATCCGCTTCCGGGTTGATGGCATCTTGCGTGAAATCGCCACACCTCCGCTGGCAATCAAGGAAAAAGTT
GCCTCCCGGATCAAGGTCATTTCCCGCCTGAATATTGCCGAGAAGCGCGTCCCCCAGGACGGTCGGATGCGACTGGTCTT
ATCCAAGACACGGGCGATCGATTTCCGGGTCAGCACACTGCCCACGCTGCAGGGCGAGAAAATTGTGCTGCGTATTCTTG
ACCCGACTTCGGCGACCTTGGGTATCGAAGCCCTCGGCTACGAGCCGGAACAGAAAGTTGCCCTGCTCGATGCCGTCAAC
CGTCCATACGGCATGGTTCTGGTAACCGGACCGACCGGCTCCGGCAAGACCGTTTCGCTTTACACCTGCCTGAATATCCT
GAACAAGGACGGGGTTAACATTTCGACCGCCGAAGACCCGGCCGAAATCAATCTGCCTGGCGTCAATCAGGTCAACGTCG
ACGACCGCGCCGGTCTAACCTTCCCCGTCGCACTCAAGGCATTCCTGCGGCAGGATCCGGACATCATCATGGTCGGCGAA
ATCCGCGACCTCGAAACGGCCGAAATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTACTGTCCACCCTACACACCAA
CGACGCCCCGCAGACGCTGACCCGTTTGATGAACATGGGGGTTCCGACCTTCAACATTGCTTCCAGCATCCAGTTGATCA
CGGCGCAGCGGCTGGCCCGGCGCCTATGCAATTGCAAAAAGCCAATGACCGTACCGAACCAGGCCCTGCTTGACGCTGGT
TACAAGGAGGCTGACCTAGATGGCTCCTGGACCCTCTATGGTCCCGGCGGCTGCGATCGGTGCAAGGGCAGCGGCTTCAA
GGGACGGGTCGGCATCTATCAGGTAATGCCGATCTCCGAAGCCATGCAGCGCTTGATCATGAGCGGCGCCAATTCCCTCG
ACCTCGCCCGCCAAGCCAAGGCAGAAGGTGTCAAGGATTTGCGCGAATCAGGATTGCTTAAAGTAAAACAGGGCGTGACC
TCGCTTGACGAGGTATTGAGCACGACCAACGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.72

100

0.548
  pilB Acinetobacter baumannii D1279779

54.007

100

0.543
  pilB Legionella pneumophila strain ERS1305867

52.668

100

0.536
  pilF Neisseria gonorrhoeae MS11

50.707

99.124

0.503
  pilB Vibrio cholerae strain A1552

48.227

98.774

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.043

98.424

0.473
  pilB Vibrio campbellii strain DS40M4

47.509

98.424

0.468
  pilF Thermus thermophilus HB27

37.919

99.299

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.567

97.898

0.368