Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AVAEIV_RS08300 Genome accession   NZ_CP092752
Coordinates   1727240..1728949 (+) Length   569 a.a.
NCBI ID   WP_302228552.1    Uniprot ID   -
Organism   Azotobacter vinelandii strain AEIV     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1722240..1733949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVAEIV_RS08275 (AVAEIV_001653) - 1723345..1724361 (-) 1017 WP_012699615.1 IS110-like element ISAzvi2 family transposase -
  AVAEIV_RS08280 (AVAEIV_001654) - 1724570..1724749 (-) 180 Protein_1628 IS3 family transposase -
  AVAEIV_RS08285 (AVAEIV_001655) istA 1724836..1725591 (+) 756 Protein_1629 IS21 family transposase -
  AVAEIV_RS08290 (AVAEIV_05275) - 1725591..1725959 (+) 369 Protein_1630 transposase -
  AVAEIV_RS08300 (AVAEIV_001657) pilF 1727240..1728949 (+) 1710 WP_302228552.1 GspE/PulE family protein Machinery gene
  AVAEIV_RS08305 (AVAEIV_001658) cysC 1728965..1729558 (+) 594 WP_012700248.1 adenylyl-sulfate kinase -
  AVAEIV_RS08310 (AVAEIV_001659) - 1729555..1730967 (+) 1413 WP_302228553.1 FAD/NAD(P)-binding protein -
  AVAEIV_RS08315 (AVAEIV_001660) - 1730975..1732249 (+) 1275 WP_231876247.1 hypothetical protein -
  AVAEIV_RS08320 (AVAEIV_001661) - 1732234..1733340 (+) 1107 WP_012700252.1 DUF1972 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62447.93 Da        Isoelectric Point: 6.3806

>NTDB_id=568112 AVAEIV_RS08300 WP_302228552.1 1727240..1728949(+) (pilF) [Azotobacter vinelandii strain AEIV]
MGVPWIRAFPLVFRGVAMRLGERLVRAGLVTEDDVQRALGLQQQAGGRLGSIFIRMGALSEDALLAVLHEQLGLPVLDGA
GMPAPSTAYAFMSGLPLRLDWLLDNNVVLWEEAGRVHVLAKDPNDSRIGDLLGYTFGDRELVRYLARAQDVDTLVDQVRR
ESAVSDLFRDDRQTLKSLIEEAPVVELVNNLLAQAVDSGASDIHVEPEETRFTVRMRVDGVLHTRMVQPYERYPAIGSRI
KLIAGLDIAEKRLPQDGRITLRLSGKDMDIRVSTAPGVFGESIVMRLLPKNRGALSLGSLGFESDHIELLRQWLAFPNGI
VLVTGPTGSGKSTTLYSALEEMRDGSNKIITVEDPVEYQVPGVTQIQAHAEIGYTFARALRAILRQDPDTIMIGEIRDLD
TAQIAIQSALTGHLVLSTLHTNDAVSAFTRLIDMGLEPFLVAASVRGVQAQRLVRKLCGHCAVRDEAPALPAAWQAMGRR
VAESDWKRACGCAHCHQTGYRGRMGIYELVPLSASLQQLVNRQAPLQEMKALIKGQGHRGLLEDGLIKASKGLTSIEEVM
RVACVEQEF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=568112 AVAEIV_RS08300 WP_302228552.1 1727240..1728949(+) (pilF) [Azotobacter vinelandii strain AEIV]
ATGGGCGTCCCGTGGATTAGGGCTTTCCCGCTGGTATTCCGAGGGGTTGCGATGAGATTGGGTGAACGGCTGGTCCGGGC
CGGGCTGGTCACTGAGGATGATGTGCAGCGCGCCTTGGGGTTGCAGCAGCAGGCGGGTGGCCGGCTCGGCTCCATTTTCA
TTCGCATGGGAGCGCTTTCCGAGGATGCCTTGCTGGCTGTGCTGCATGAGCAGTTGGGGTTGCCGGTTCTGGACGGGGCC
GGGATGCCGGCCCCGTCCACTGCCTACGCCTTCATGTCCGGTCTTCCGTTACGGCTGGACTGGTTGCTCGACAACAATGT
AGTGCTGTGGGAAGAGGCCGGCAGGGTGCACGTATTGGCCAAGGACCCGAACGATTCGCGGATCGGGGATCTGCTGGGCT
ACACCTTCGGTGACCGGGAACTGGTGCGCTATCTGGCCCGCGCTCAGGATGTCGACACGCTCGTCGATCAGGTGCGCAGG
GAGAGCGCCGTATCCGATCTGTTTCGCGATGATCGCCAGACCCTCAAGAGCCTGATCGAAGAAGCGCCGGTCGTCGAGCT
GGTGAACAATCTCCTGGCGCAGGCGGTCGATTCGGGAGCTTCCGACATTCATGTCGAGCCCGAGGAGACCCGTTTTACCG
TGCGGATGCGAGTCGACGGCGTTCTGCACACGCGCATGGTGCAGCCTTACGAGCGTTATCCGGCGATCGGTTCGCGCATC
AAGCTGATCGCCGGGCTGGATATCGCCGAGAAGCGTTTGCCGCAGGATGGCCGGATTACCTTGCGCCTGAGCGGAAAGGA
CATGGATATCCGGGTTTCCACGGCGCCGGGAGTTTTCGGCGAGTCGATCGTCATGCGGTTGTTGCCGAAGAACCGTGGTG
CCCTGTCGCTGGGCAGCCTGGGTTTCGAGTCCGACCATATCGAGTTGCTCAGGCAGTGGCTGGCATTTCCTAATGGCATT
GTGCTAGTAACTGGGCCCACGGGGTCCGGCAAGTCCACGACCCTGTATTCGGCGCTCGAGGAGATGCGCGATGGCAGCAA
CAAGATCATCACCGTGGAGGACCCCGTCGAGTATCAGGTACCGGGCGTGACGCAGATCCAGGCGCACGCGGAAATCGGCT
ACACCTTCGCTCGTGCGCTGCGGGCCATTCTGCGCCAGGACCCGGACACCATCATGATCGGTGAGATTCGCGACCTGGAT
ACCGCGCAGATCGCCATCCAGTCCGCTTTGACAGGGCACCTGGTGTTGTCGACGCTGCATACCAATGACGCGGTTTCGGC
CTTCACCCGCCTGATCGACATGGGGTTGGAGCCTTTTCTGGTGGCCGCTTCGGTGCGCGGTGTTCAGGCGCAGCGTCTGG
TGCGCAAGCTCTGCGGGCATTGCGCGGTGCGCGACGAGGCGCCGGCCTTGCCGGCTGCCTGGCAGGCAATGGGACGTCGC
GTCGCGGAGAGCGACTGGAAACGGGCATGCGGTTGTGCCCATTGCCACCAGACCGGCTATCGCGGTCGCATGGGGATATA
CGAGTTGGTACCCCTGAGCGCTTCCCTGCAGCAGTTGGTCAATCGCCAGGCGCCTCTGCAGGAGATGAAGGCGCTGATCA
AGGGTCAGGGGCACCGGGGCCTGCTGGAGGATGGATTGATCAAGGCCAGCAAGGGTCTGACCAGTATCGAGGAGGTCATG
CGTGTTGCTTGTGTCGAACAGGAATTCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-161)

Type II/IV secretion system protein (T2SSE)

(190-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.899

98.946

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.177

98.243

0.385