Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MJ614_RS18515 Genome accession   NZ_CP092647
Coordinates   3383738..3384178 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain EC5502     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3378738..3389178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ614_RS18490 coaE 3379228..3379848 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  MJ614_RS18495 - 3379873..3379917 (+) 45 WP_120795372.1 protein YacM -
  MJ614_RS18500 guaC 3380073..3381116 (+) 1044 WP_001217338.1 GMP reductase -
  MJ614_RS18505 hofC 3381151..3382353 (-) 1203 WP_000157234.1 protein transport protein HofC -
  MJ614_RS18510 pilB 3382343..3383728 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  MJ614_RS18515 pilA 3383738..3384178 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  MJ614_RS18520 nadC 3384381..3385274 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MJ614_RS18525 ampD 3385362..3385913 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MJ614_RS18530 ampE 3385910..3386764 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MJ614_RS18535 aroP 3386807..3388180 (-) 1374 WP_001331235.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=567728 MJ614_RS18515 WP_000360895.1 3383738..3384178(-) (pilA) [Escherichia coli strain EC5502]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=567728 MJ614_RS18515 WP_000360895.1 3383738..3384178(-) (pilA) [Escherichia coli strain EC5502]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363