Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MJ614_RS12845 Genome accession   NZ_CP092647
Coordinates   2167876..2169372 (-) Length   498 a.a.
NCBI ID   WP_001040668.1    Uniprot ID   -
Organism   Escherichia coli strain EC5502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2162876..2174372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ614_RS12820 panM 2163098..2163481 (+) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
  MJ614_RS12825 livJ 2163680..2164783 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  MJ614_RS12830 rpoH 2165055..2165909 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  MJ614_RS12835 ftsX 2166154..2167212 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  MJ614_RS12840 ftsE 2167205..2167873 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  MJ614_RS12845 pilA 2167876..2169372 (-) 1497 WP_001040668.1 signal recognition particle-docking protein FtsY Machinery gene
  MJ614_RS12850 rsmD 2169522..2170118 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  MJ614_RS12855 yhhL 2170108..2170377 (+) 270 WP_001295207.1 DUF1145 family protein -
  MJ614_RS12860 yhhM 2170380..2170739 (-) 360 WP_000042901.1 DUF2500 domain-containing protein -
  MJ614_RS12865 yhhN 2170880..2171506 (+) 627 WP_000964718.1 lysoplasmalogenase -
  MJ614_RS12870 zntA 2171580..2173778 (+) 2199 WP_000106527.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  MJ614_RS12875 tusA 2173880..2174125 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54655.50 Da        Isoelectric Point: 4.1560

>NTDB_id=567700 MJ614_RS12845 WP_001040668.1 2167876..2169372(-) (pilA) [Escherichia coli strain EC5502]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPVPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=567700 MJ614_RS12845 WP_001040668.1 2167876..2169372(-) (pilA) [Escherichia coli strain EC5502]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCAGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGTGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAGGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCTTGCAGAACAAATCGCACCTGATGGAAGAGCTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375