Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KH277_RS17680 Genome accession   NZ_CP075054
Coordinates   3614198..3614635 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain VTX20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3609198..3619635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH277_RS17665 (KH277_17655) - 3612056..3612418 (-) 363 WP_003156585.1 helix-turn-helix domain-containing protein -
  KH277_RS17670 (KH277_17660) hxlA 3612650..3613285 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  KH277_RS17675 (KH277_17665) hxlB 3613282..3613839 (+) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  KH277_RS17680 (KH277_17670) nucA/comI 3614198..3614635 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KH277_RS17685 (KH277_17675) nin/comJ 3614657..3615055 (+) 399 WP_014304317.1 competence protein ComJ Regulator
  KH277_RS17690 (KH277_17680) - 3615096..3615314 (-) 219 Protein_3476 family 1 glycosylhydrolase -
  KH277_RS17700 (KH277_17690) - 3615533..3615853 (-) 321 WP_015388719.1 YckD family protein -
  KH277_RS17705 (KH277_17695) - 3615925..3616359 (-) 435 WP_003156592.1 RDD family protein -
  KH277_RS17710 (KH277_17700) - 3616374..3617510 (-) 1137 WP_052827030.1 zinc-dependent alcohol dehydrogenase -
  KH277_RS17715 (KH277_17705) - 3617575..3618777 (-) 1203 WP_171463421.1 GTP-binding protein -
  KH277_RS19325 - 3618930..3619055 (+) 126 Protein_3481 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=567505 KH277_RS17680 WP_003156588.1 3614198..3614635(+) (nucA/comI) [Bacillus velezensis strain VTX20]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=567505 KH277_RS17680 WP_003156588.1 3614198..3614635(+) (nucA/comI) [Bacillus velezensis strain VTX20]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641