Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KH277_RS01985 Genome accession   NZ_CP075054
Coordinates   387037..388182 (-) Length   381 a.a.
NCBI ID   WP_088037228.1    Uniprot ID   -
Organism   Bacillus velezensis strain VTX20     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 382037..393182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH277_RS01945 (KH277_01945) speE 382328..383158 (+) 831 WP_003151052.1 spermidine synthase -
  KH277_RS01950 (KH277_01950) speB 383218..384090 (+) 873 WP_003151054.1 agmatinase -
  KH277_RS01955 (KH277_01955) - 384261..384479 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  KH277_RS01960 (KH277_01960) - 385070..385582 (-) 513 WP_003151059.1 stage II sporulation protein M -
  KH277_RS01965 (KH277_01965) - 385626..385907 (-) 282 WP_003151060.1 hypothetical protein -
  KH277_RS19190 - 386095..386280 (-) 186 Protein_379 DUF4177 domain-containing protein -
  KH277_RS01970 (KH277_01970) - 386315..386518 (-) 204 WP_003151062.1 hypothetical protein -
  KH277_RS01975 (KH277_01975) - 386532..386735 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  KH277_RS01980 (KH277_01980) - 386934..387053 (-) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  KH277_RS01985 (KH277_01985) rapF 387037..388182 (-) 1146 WP_088037228.1 tetratricopeptide repeat protein Regulator
  KH277_RS01990 (KH277_01990) - 388474..388902 (+) 429 WP_003151069.1 DUF1934 family protein -
  KH277_RS01995 (KH277_01995) argS 388899..390569 (+) 1671 WP_003151071.1 arginine--tRNA ligase -
  KH277_RS02000 (KH277_02000) narK 390704..391894 (+) 1191 WP_003151073.1 nitrate transporter NarK -
  KH277_RS02005 (KH277_02005) - 391992..392708 (+) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45325.46 Da        Isoelectric Point: 4.9264

>NTDB_id=567443 KH277_RS01985 WP_088037228.1 387037..388182(-) (rapF) [Bacillus velezensis strain VTX20]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=567443 KH277_RS01985 WP_088037228.1 387037..388182(-) (rapF) [Bacillus velezensis strain VTX20]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGACTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.541

100

0.745
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53