Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MKF35_RS13795 Genome accession   NZ_CP092629
Coordinates   2988513..2989727 (+) Length   404 a.a.
NCBI ID   WP_003112721.1    Uniprot ID   Q9I5N8
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2983513..2994727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS13780 gspM 2984106..2984699 (+) 594 WP_003112724.1 type II secretion system protein GspM -
  MKF35_RS13785 gspD 2984696..2987107 (+) 2412 WP_009315212.1 type II secretion system secretin GspD -
  MKF35_RS13790 pilB 2987104..2988513 (+) 1410 WP_003120811.1 type II secretion system ATPase GspE Machinery gene
  MKF35_RS13795 pilC 2988513..2989727 (+) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  MKF35_RS13800 lapA 2990241..2991347 (+) 1107 WP_003112123.1 alkaline phosphatase LapA -
  MKF35_RS13805 - 2991433..2992545 (+) 1113 WP_003112720.1 PstS family phosphate ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43409.42 Da        Isoelectric Point: 9.3769

>NTDB_id=567243 MKF35_RS13795 WP_003112721.1 2988513..2989727(+) (pilC) [Pseudomonas aeruginosa strain HU20]
MQTFRYEAADAQGRIETGTLEADSQRGALGQLRARGLTPLEVREQAGGGTGQGAGALFAPRLSDGDLAWATRQLASLLAA
SLPLEAALSATLDQAERKHIAQTLSAVRSDVRGGMRLADALAARPRDFPEIYRALVAAGEESGDLAQVMERLADYIEERN
ALRGKILTAFIYPAVVGVVSIGIVIFLLGYVVPQVVSAFSQARQDLPALTRAMLQASDFVRAWGWLCAGAIGSAYWGWRL
YLRDPQARLGWHRRVLRLPLLGRFVLGVNTARFASTLAILGSAGVPLLRALDAARQTLANDCLAQAVEEATAQVREGVSL
ASALRTRQVFPPILTHLIASGEKTGALPPMLDRAAQTLSRDIERRAMGMTALLEPLMIVVMGGVVLTIVMAVLMPIIEMN
QLVQ

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=567243 MKF35_RS13795 WP_003112721.1 2988513..2989727(+) (pilC) [Pseudomonas aeruginosa strain HU20]
ATGCAGACCTTCCGTTACGAAGCCGCCGACGCCCAGGGCCGGATCGAGACCGGCACCCTGGAGGCGGACAGCCAGCGTGG
CGCCCTCGGCCAACTGCGCGCCCGCGGCCTGACCCCGCTGGAGGTCCGCGAGCAGGCTGGCGGCGGGACCGGGCAAGGGG
CGGGCGCGCTGTTCGCCCCGCGCCTGTCCGACGGCGACCTGGCCTGGGCCACCCGCCAGCTGGCCAGCCTGCTGGCCGCT
AGCCTGCCGCTGGAGGCGGCGCTGAGCGCCACCCTCGACCAGGCCGAGCGCAAGCACATCGCCCAGACCCTGAGCGCCGT
GCGCAGCGACGTGCGCGGCGGCATGCGCCTGGCCGACGCCCTTGCCGCACGGCCGCGGGACTTCCCGGAAATCTACCGGG
CGCTGGTGGCTGCGGGCGAGGAGTCCGGCGACCTGGCCCAGGTGATGGAGCGCCTGGCCGACTACATCGAGGAACGCAAC
GCCCTGCGCGGCAAGATCCTCACCGCGTTCATCTACCCGGCGGTGGTCGGCGTGGTCTCCATCGGCATCGTCATTTTCCT
CCTCGGCTACGTGGTGCCGCAGGTGGTCAGCGCCTTCTCCCAGGCGCGCCAGGACCTGCCGGCGCTGACCCGGGCGATGC
TCCAGGCCAGCGACTTCGTGCGCGCCTGGGGCTGGCTCTGCGCCGGCGCCATCGGTAGCGCTTACTGGGGCTGGCGCTTG
TACCTGCGCGACCCGCAGGCGCGGCTCGGCTGGCACCGGCGAGTGCTGCGCCTGCCTCTGCTCGGTCGCTTCGTGCTCGG
GGTGAACACCGCGCGCTTCGCCTCCACCCTGGCGATCCTCGGCAGCGCCGGCGTACCGCTGCTGCGCGCGCTCGATGCGG
CGCGCCAGACGCTGGCCAACGACTGCCTCGCGCAGGCGGTGGAGGAGGCCACCGCGCAGGTTCGCGAAGGTGTCTCGCTG
GCCTCGGCGCTGCGCACGCGGCAGGTGTTCCCGCCGATCCTCACCCACCTGATCGCCAGCGGCGAGAAGACCGGTGCGCT
GCCGCCGATGCTCGACCGCGCGGCGCAGACCCTGTCGCGCGACATCGAGCGCCGCGCCATGGGCATGACCGCGTTGCTCG
AGCCGCTGATGATCGTGGTCATGGGCGGGGTGGTGCTGACCATCGTGATGGCGGTGCTGATGCCGATCATCGAGATGAAC
CAACTGGTCCAGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)

Type II secretion system (T2SS), protein F (T2SSF)

(71-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9I5N8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Thermus thermophilus HB27

36.386

100

0.364