Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   MKF35_RS09670 Genome accession   NZ_CP092629
Coordinates   2076840..2077436 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2071840..2082436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS09660 - 2073120..2075591 (-) 2472 WP_017148635.1 penicillin-binding protein 1A -
  MKF35_RS09665 pilM 2075776..2076840 (+) 1065 WP_047105348.1 type IV pilus assembly protein PilM Machinery gene
  MKF35_RS09670 pilN 2076840..2077436 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  MKF35_RS09675 pilO 2077433..2078056 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  MKF35_RS09680 comL/pilP 2078053..2078577 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  MKF35_RS09685 pilQ 2078631..2080775 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  MKF35_RS09690 aroK 2080787..2081305 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=567220 MKF35_RS09670 WP_003095839.1 2076840..2077436(+) (pilN) [Pseudomonas aeruginosa strain HU20]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=567220 MKF35_RS09670 WP_003095839.1 2076840..2077436(+) (pilN) [Pseudomonas aeruginosa strain HU20]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369