Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MKF35_RS05060 Genome accession   NZ_CP092629
Coordinates   1075431..1076783 (-) Length   450 a.a.
NCBI ID   WP_003123870.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1070431..1081783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS05025 thiS 1070665..1070865 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  MKF35_RS05030 - 1070968..1071345 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  MKF35_RS05035 mtgA 1071382..1072113 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  MKF35_RS05040 - 1072118..1072723 (-) 606 WP_003084511.1 hypothetical protein -
  MKF35_RS05045 rpoH 1072788..1073642 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  MKF35_RS05050 ftsX 1073756..1074763 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  MKF35_RS05055 ftsE 1074763..1075434 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  MKF35_RS05060 pilA 1075431..1076783 (-) 1353 WP_003123870.1 signal recognition particle-docking protein FtsY Machinery gene
  MKF35_RS05065 - 1076904..1078301 (+) 1398 WP_003084498.1 pitrilysin family protein -
  MKF35_RS05070 - 1078294..1079781 (+) 1488 WP_047105395.1 pitrilysin family protein -
  MKF35_RS05075 rsmD 1079781..1080377 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  MKF35_RS05080 - 1080380..1080946 (+) 567 WP_003117889.1 hypothetical protein -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47872.27 Da        Isoelectric Point: 4.5795

>NTDB_id=567197 MKF35_RS05060 WP_003123870.1 1075431..1076783(-) (pilA) [Pseudomonas aeruginosa strain HU20]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=567197 MKF35_RS05060 WP_003123870.1 1075431..1076783(-) (pilA) [Pseudomonas aeruginosa strain HU20]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCTCTGGCCGCCGCTCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGC
GAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(245-444)

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96

0.456