Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MKI52_RS07820 Genome accession   NZ_CP092581
Coordinates   1632589..1634862 (-) Length   757 a.a.
NCBI ID   WP_000595012.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VRMSSA-WC111     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1627589..1639862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKI52_RS07800 (MKI52_001545) - 1628096..1628971 (-) 876 WP_000717808.1 N-acetylmuramoyl-L-alanine amidase -
  MKI52_RS07805 (MKI52_001546) dtd 1628968..1629420 (-) 453 WP_000869983.1 D-aminoacyl-tRNA deacylase -
  MKI52_RS07810 (MKI52_001547) - 1629432..1631621 (-) 2190 WP_001058587.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MKI52_RS07815 (MKI52_001548) - 1632049..1632567 (-) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  MKI52_RS07820 (MKI52_001549) recJ 1632589..1634862 (-) 2274 WP_000595012.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MKI52_RS07825 (MKI52_001550) secDF 1635065..1637344 (-) 2280 WP_000749795.1 protein translocase subunit SecDF -
  MKI52_RS07830 (MKI52_001551) yajC 1637619..1637879 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -
  MKI52_RS07835 (MKI52_001552) tgt 1637898..1639037 (-) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85583.83 Da        Isoelectric Point: 4.8732

>NTDB_id=567149 MKI52_RS07820 WP_000595012.1 1632589..1634862(-) (recJ) [Staphylococcus aureus strain VRMSSA-WC111]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDVNHDALQLSDMTKAIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKKGDLFLLLVKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=567149 MKI52_RS07820 WP_000595012.1 1632589..1634862(-) (recJ) [Staphylococcus aureus strain VRMSSA-WC111]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATTGAATCCATCATTTCAGACACAG
ATGTCAATCATGATGCATTGCAATTGAGCGATATGACTAAGGCTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAGGGAGATTTATTTTTACTTTT
AGTAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCCAGTAGACG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATCGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCAACGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(452-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

DHHA1 domain (DHHA1)

(312-436)

DHH family (DHH)

(81-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.015

100

0.39
  recJ Streptococcus pneumoniae strain D39V

38.076

100

0.382