Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AZKH_RS13500 Genome accession   NC_020516
Coordinates   3092623..3094005 (-) Length   460 a.a.
NCBI ID   WP_015436340.1    Uniprot ID   H0PSU3
Organism   Azoarcus sp. KH32C     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3087623..3099005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS13490 (AZKH_2727) - 3090589..3090987 (-) 399 WP_015436338.1 response regulator -
  AZKH_RS13495 (AZKH_2728) - 3090991..3092472 (-) 1482 WP_015436339.1 PAS domain S-box protein -
  AZKH_RS13500 (AZKH_2729) radA 3092623..3094005 (-) 1383 WP_015436340.1 DNA repair protein RadA Machinery gene
  AZKH_RS13505 (AZKH_2730) - 3094005..3094922 (-) 918 WP_015436341.1 LysR family transcriptional regulator -
  AZKH_RS13510 (AZKH_2731) lplT 3095101..3096351 (+) 1251 WP_015436342.1 lysophospholipid transporter LplT -
  AZKH_RS13515 (AZKH_2732) - 3096373..3097224 (-) 852 WP_015436343.1 uracil-DNA glycosylase family protein -
  AZKH_RS13520 (AZKH_2733) rimI 3097221..3097676 (-) 456 WP_015436344.1 ribosomal protein S18-alanine N-acetyltransferase -
  AZKH_RS13525 (AZKH_2734) tsaB 3097673..3098365 (-) 693 WP_015436345.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  AZKH_RS27305 (AZKH_2735) - 3098454..3098831 (-) 378 WP_156822109.1 hypothetical protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 48899.25 Da        Isoelectric Point: 7.1218

>NTDB_id=56714 AZKH_RS13500 WP_015436340.1 3092623..3094005(-) (radA) [Azoarcus sp. KH32C]
MAKAKTAYVCTECGGQAPRWQGQCPQCRAWNTLVESIVEPVATAATNRFAALAGATGKLQTLAELEPREEPRTPTGIEEF
DRVLGGGLVAGGVVLIGGDPGIGKSTLLLQALAVLAGEHAAVYVSGEESGEQVALRAQRLQLDPGSLQLLPEINLERILA
TLKNTAPRIAVIDSIQTVYSEALQSAPGSVAQVRECAAQLTRFAKQSGTSLIMVGHVTKDGTLAGPRVLEHIVDTVLYFE
GDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVSNPSAIFLSQHSQEVAGSCVLVTQEGTRPLLVEIQALVDSAHSP
NPRRLSVGLEQNRLAMLLAVLHRHAGVVCFDQDVFVNAVGGVKISEPAADLAVLLAIVSSLRSRALPRGLVVFGEVGLAG
EIRPAPRGQERLKEAAKLGFTVAIIPRANAPKQPISGMRVIPVDRIEEAIEQARELEARS

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=56714 AZKH_RS13500 WP_015436340.1 3092623..3094005(-) (radA) [Azoarcus sp. KH32C]
ATGGCGAAGGCGAAGACGGCGTACGTCTGCACCGAATGCGGTGGGCAGGCGCCGCGCTGGCAGGGGCAGTGTCCTCAATG
CCGGGCCTGGAACACGCTCGTCGAGTCGATCGTCGAGCCGGTCGCCACGGCCGCTACGAATCGCTTTGCAGCTCTGGCTG
GTGCGACCGGCAAACTGCAGACGCTGGCCGAACTCGAACCGCGGGAGGAGCCGCGTACGCCGACCGGGATCGAGGAATTC
GACCGCGTGCTGGGCGGCGGACTCGTTGCGGGCGGCGTCGTGCTGATCGGGGGCGATCCCGGGATCGGCAAGTCGACGCT
GCTGTTGCAGGCGCTAGCCGTGCTTGCCGGGGAGCATGCGGCGGTTTACGTGAGCGGCGAGGAGTCCGGGGAACAGGTCG
CGCTGCGCGCGCAACGACTTCAGCTCGATCCAGGCAGCCTGCAGTTGCTGCCCGAGATCAACCTCGAACGCATCCTCGCC
ACGCTCAAGAACACGGCGCCGCGCATTGCCGTGATCGATTCGATCCAGACCGTGTATTCGGAGGCCTTACAGTCGGCGCC
GGGCTCGGTGGCGCAGGTGCGCGAATGTGCGGCGCAGCTCACGCGTTTCGCGAAGCAGAGCGGCACGAGCCTCATCATGG
TCGGCCACGTGACGAAGGACGGCACGCTCGCGGGGCCGCGCGTGCTGGAGCATATCGTCGATACCGTGCTGTATTTCGAG
GGCGACACGCATTCCAGTTTCCGCCTGGTGCGCGCATTCAAGAACCGCTTCGGCGCGGTGAATGAGCTCGGCGTGTTCGC
GATGACCGAACGCGGACTGCGCGGGGTGAGCAATCCGTCGGCGATCTTCCTGTCGCAGCATAGCCAGGAGGTGGCGGGCA
GTTGCGTGCTGGTGACGCAGGAAGGCACGCGCCCGTTGCTGGTCGAGATCCAGGCGCTGGTCGACAGCGCCCACAGTCCG
AATCCGCGGCGTCTGTCGGTGGGACTCGAGCAGAACCGTCTGGCGATGCTGCTCGCGGTGCTGCACCGCCACGCCGGCGT
TGTGTGCTTCGATCAGGATGTCTTCGTCAATGCGGTCGGCGGCGTGAAGATCAGCGAACCGGCTGCGGACCTTGCGGTTC
TGCTGGCGATCGTGTCGTCGCTGCGCAGCCGCGCCTTGCCACGCGGGCTCGTCGTGTTCGGCGAAGTAGGCCTCGCGGGC
GAGATCCGGCCGGCGCCGCGCGGGCAGGAACGGTTGAAGGAAGCGGCGAAGCTGGGTTTCACTGTGGCGATCATCCCACG
GGCGAATGCGCCCAAGCAGCCGATCTCCGGCATGCGGGTGATCCCGGTCGACCGGATCGAGGAAGCGATCGAACAGGCGC
GCGAGCTCGAAGCCCGTTCGTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(360-453)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(81-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0PSU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.891

99.783

0.498
  radA Streptococcus pneumoniae Rx1

45.514

99.348

0.452
  radA Streptococcus pneumoniae D39

45.514

99.348

0.452
  radA Streptococcus pneumoniae R6

45.514

99.348

0.452
  radA Streptococcus pneumoniae TIGR4

45.514

99.348

0.452
  radA Streptococcus mitis SK321

45.695

98.478

0.45
  radA Streptococcus mitis NCTC 12261

45.475

98.478

0.448

Multiple sequence alignment