Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JRY14_RS23555 Genome accession   NZ_CP074714
Coordinates   4348601..4349644 (-) Length   347 a.a.
NCBI ID   WP_086412120.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain B13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343601..4354644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JRY14_RS23510 (JRY14_23480) - 4344035..4344235 (-) 201 WP_000106083.1 YqzE family protein -
  JRY14_RS23515 (JRY14_23485) aroK 4344274..4344771 (-) 498 WP_000836624.1 shikimate kinase AroK -
  JRY14_RS23520 (JRY14_23490) - 4344887..4345537 (-) 651 WP_000183867.1 2OG-Fe(II) oxygenase -
  JRY14_RS23525 (JRY14_23495) comGG 4345712..4346083 (-) 372 WP_001231318.1 competence type IV pilus minor pilin ComGG -
  JRY14_RS23530 (JRY14_23500) comGF 4346080..4346550 (-) 471 WP_000923106.1 competence type IV pilus minor pilin ComGF -
  JRY14_RS23535 (JRY14_23505) - 4346520..4346821 (-) 302 Protein_4345 type II secretion system protein -
  JRY14_RS23540 (JRY14_23510) comGD 4346814..4347269 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  JRY14_RS23545 (JRY14_23515) comGC 4347266..4347565 (-) 300 WP_001178684.1 competence type IV pilus major pilin ComGC -
  JRY14_RS23550 (JRY14_23520) comGB 4347577..4348614 (-) 1038 WP_016123380.1 competence type IV pilus assembly protein ComGB -
  JRY14_RS23555 (JRY14_23525) comGA 4348601..4349644 (-) 1044 WP_086412120.1 competence type IV pilus ATPase ComGA Machinery gene
  JRY14_RS23560 (JRY14_23530) - 4349850..4350545 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  JRY14_RS23565 (JRY14_23535) - 4350674..4350916 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  JRY14_RS23570 (JRY14_23540) - 4351024..4352418 (+) 1395 WP_025709781.1 L-cystine transporter -
  JRY14_RS23575 (JRY14_23545) - 4352498..4352890 (-) 393 WP_000875399.1 hypothetical protein -
  JRY14_RS23580 (JRY14_23550) - 4352978..4353193 (-) 216 WP_001008327.1 DUF3912 family protein -
  JRY14_RS23585 (JRY14_23555) - 4353446..4353757 (+) 312 WP_001093237.1 hypothetical protein -
  JRY14_RS23590 (JRY14_23560) - 4353792..4354280 (-) 489 WP_140159380.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39124.53 Da        Isoelectric Point: 8.3536

>NTDB_id=565988 JRY14_RS23555 WP_086412120.1 4348601..4349644(-) (comGA) [Bacillus thuringiensis strain B13]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTIQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAMIRFIDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAEGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=565988 JRY14_RS23555 WP_086412120.1 4348601..4349644(-) (comGA) [Bacillus thuringiensis strain B13]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGATACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCCACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGTCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATGATTCGTTTCATAGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGAGGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556