Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JRY14_RS22290 Genome accession   NZ_CP074714
Coordinates   4128763..4129197 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain B13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4123763..4134197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JRY14_RS22275 (JRY14_22245) - 4124940..4125497 (+) 558 WP_000062062.1 PadR family transcriptional regulator -
  JRY14_RS22280 (JRY14_22250) metE 4126010..4128298 (+) 2289 WP_086412205.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  JRY14_RS22285 (JRY14_22255) comJ 4128337..4128726 (-) 390 WP_000424030.1 competence protein ComJ -
  JRY14_RS22290 (JRY14_22260) nucA/comI 4128763..4129197 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  JRY14_RS22295 (JRY14_22265) - 4129424..4130173 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  JRY14_RS22300 (JRY14_22270) kinB 4130212..4131486 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  JRY14_RS22305 (JRY14_22275) - 4131797..4131997 (+) 201 WP_000929280.1 hypothetical protein -
  JRY14_RS22310 (JRY14_22280) - 4132120..4133283 (+) 1164 WP_000434577.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=565985 JRY14_RS22290 WP_000811494.1 4128763..4129197(-) (nucA/comI) [Bacillus thuringiensis strain B13]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=565985 JRY14_RS22290 WP_000811494.1 4128763..4129197(-) (nucA/comI) [Bacillus thuringiensis strain B13]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549