Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IS509_RS04435 Genome accession   NZ_CP074698
Coordinates   927346..928464 (-) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain X4-136     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 886071..952971 927346..928464 within 0


Gene organization within MGE regions


Location: 886071..952971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS509_RS04230 - 886706..888331 (-) 1626 WP_000834616.1 NAD+ synthase -
  IS509_RS04235 - 888716..889276 (-) 561 WP_001048150.1 hypothetical protein -
  IS509_RS04240 - 889367..890227 (-) 861 WP_000838098.1 pirin family protein -
  IS509_RS04245 hemJ 890398..890850 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  IS509_RS04250 - 890869..892098 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  IS509_RS04255 - 892395..893720 (+) 1326 WP_000160011.1 EcsC family protein -
  IS509_RS04260 rpsL 893921..894295 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  IS509_RS04265 rpsG 894455..894925 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  IS509_RS04270 fusA 895104..897242 (+) 2139 WP_000113824.1 elongation factor G -
  IS509_RS04275 tuf 897337..898527 (+) 1191 WP_001029610.1 elongation factor Tu -
  IS509_RS04280 - 898731..899612 (+) 882 WP_000992305.1 metal-dependent hydrolase -
  IS509_RS04285 - 899849..900724 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  IS509_RS04290 rimI 900833..901288 (+) 456 WP_000621543.1 ribosomal protein S18-alanine N-acetyltransferase -
  IS509_RS04295 - 901333..902148 (-) 816 WP_000844343.1 arginyltransferase -
  IS509_RS04300 aat 902167..902898 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IS509_RS04305 trxB 903018..903965 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  IS509_RS04310 - 904235..907267 (+) 3033 WP_000127823.1 DNA translocase FtsK -
  IS509_RS04315 rhtC 907417..908037 (+) 621 WP_000959259.1 threonine export protein RhtC -
  IS509_RS04320 - 908093..908515 (-) 423 Protein_826 hypothetical protein -
  IS509_RS04325 - 908549..910195 (-) 1647 WP_001094416.1 IS66-like element ISAba17 family transposase -
  IS509_RS04330 tnpB 910270..910605 (-) 336 WP_000618090.1 IS66 family insertion sequence element accessory protein TnpB -
  IS509_RS04335 - 910602..911030 (-) 429 WP_000364662.1 transposase -
  IS509_RS04340 - 911112..911240 (-) 129 Protein_830 hypothetical protein -
  IS509_RS04345 - 911652..911939 (-) 288 WP_001218560.1 PA4642 family protein -
  IS509_RS04350 minE 912023..912295 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IS509_RS04355 minD 912298..913110 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  IS509_RS04360 minC 913181..913903 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  IS509_RS04365 - 914025..915071 (-) 1047 WP_001181639.1 hypothetical protein -
  IS509_RS04370 - 915088..916014 (-) 927 WP_000100965.1 acyltransferase -
  IS509_RS04375 - 916259..916912 (+) 654 WP_001202415.1 OmpA family protein -
  IS509_RS04380 rep 917106..919145 (+) 2040 WP_000093035.1 DNA helicase Rep -
  IS509_RS04385 dut 919170..919622 (+) 453 WP_000868152.1 dUTP diphosphatase -
  IS509_RS04390 - 919822..921240 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  IS509_RS04395 argB 921255..922163 (+) 909 WP_001135419.1 acetylglutamate kinase -
  IS509_RS04400 - 922284..923066 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  IS509_RS04405 - 923175..924023 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  IS509_RS04410 - 924276..924731 (+) 456 WP_000782976.1 bacteriohemerythrin -
  IS509_RS04415 - 924884..925951 (+) 1068 WP_002019572.1 hypothetical protein -
  IS509_RS04420 - 925951..926274 (+) 324 WP_000442587.1 RnfH family protein -
  IS509_RS04425 bamE 926329..926727 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  IS509_RS04430 fur 926839..927276 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  IS509_RS04435 pilU 927346..928464 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IS509_RS04440 pilT 928492..929529 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  IS509_RS04445 - 929656..930348 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  IS509_RS04450 - 930550..934146 (-) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  IS509_RS04455 - 934156..935424 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  IS509_RS04460 - 935588..936061 (+) 474 WP_000974358.1 OsmC family protein -
  IS509_RS04465 - 936129..936506 (-) 378 WP_001059438.1 VOC family protein -
  IS509_RS04470 - 936886..937338 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  IS509_RS04475 - 937428..938333 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  IS509_RS04480 - 938325..939440 (-) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  IS509_RS04485 hemB 939466..940479 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  IS509_RS04490 - 940622..941119 (+) 498 WP_002019577.1 thioesterase family protein -
  IS509_RS04495 - 941389..942540 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IS509_RS04500 - 942547..944070 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  IS509_RS04505 ggt 944373..946358 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  IS509_RS04515 - 947060..948289 (+) 1230 WP_001991065.1 tyrosine-type recombinase/integrase -
  IS509_RS04520 - 948437..949204 (+) 768 WP_000578618.1 hypothetical protein -
  IS509_RS04525 - 949414..949629 (+) 216 WP_000153154.1 hypothetical protein -
  IS509_RS04530 - 949632..949841 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  IS509_RS04535 - 949838..950392 (+) 555 WP_001988245.1 BRO family protein -
  IS509_RS04540 - 950385..950753 (+) 369 WP_001046481.1 hypothetical protein -
  IS509_RS04545 - 950763..951065 (+) 303 Protein_870 hypothetical protein -
  IS509_RS04550 - 951193..951471 (+) 279 WP_001064707.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=565856 IS509_RS04435 WP_000347039.1 927346..928464(-) (pilU) [Acinetobacter baumannii strain X4-136]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=565856 IS509_RS04435 WP_000347039.1 927346..928464(-) (pilU) [Acinetobacter baumannii strain X4-136]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395