Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MKD44_RS19125 Genome accession   NZ_CP092534
Coordinates   3928155..3928595 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain DS566     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3923155..3933595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKD44_RS19105 (MKD44_19100) aroP 3924155..3925525 (+) 1371 WP_001540704.1 aromatic amino acid transporter AroP -
  MKD44_RS19110 (MKD44_19105) ampE 3925568..3926422 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  MKD44_RS19115 (MKD44_19110) ampD 3926419..3926970 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MKD44_RS19120 (MKD44_19115) nadC 3927058..3927951 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MKD44_RS19125 (MKD44_19120) pilA 3928155..3928595 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  MKD44_RS19130 (MKD44_19125) pilB 3928605..3929990 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  MKD44_RS19135 (MKD44_19130) hofC 3929980..3931182 (+) 1203 WP_001540700.1 protein transport protein HofC -
  MKD44_RS19140 (MKD44_19135) guaC 3931217..3932260 (-) 1044 WP_001217338.1 GMP reductase -
  MKD44_RS19145 (MKD44_19140) - 3932416..3932460 (-) 45 WP_120795372.1 protein YacM -
  MKD44_RS19150 (MKD44_19145) coaE 3932485..3933105 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=565813 MKD44_RS19125 WP_000360914.1 3928155..3928595(+) (pilA) [Escherichia coli strain DS566]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=565813 MKD44_RS19125 WP_000360914.1 3928155..3928595(+) (pilA) [Escherichia coli strain DS566]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363