Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KH389_RS25330 Genome accession   NZ_CP074676
Coordinates   5537936..5539216 (-) Length   426 a.a.
NCBI ID   WP_420831945.1    Uniprot ID   -
Organism   Pseudomonas qingdaonensis strain S-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5532936..5544216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH389_RS25315 (KH389_25315) - 5534603..5536198 (-) 1596 WP_043859937.1 AAA family ATPase -
  KH389_RS25320 (KH389_25320) aroB 5536215..5537312 (-) 1098 WP_043859938.1 3-dehydroquinate synthase -
  KH389_RS25325 (KH389_25325) aroK 5537413..5537931 (-) 519 WP_043859939.1 shikimate kinase AroK -
  KH389_RS25330 (KH389_25330) pilQ 5537936..5539216 (-) 1281 WP_420831945.1 type IV pilus secretin PilQ Machinery gene
  KH389_RS25335 (KH389_25335) - 5539232..5540137 (-) 906 WP_213606460.1 pilus assembly protein PilP -
  KH389_RS25340 (KH389_25340) - 5540134..5540664 (-) 531 WP_213606462.1 PilN domain-containing protein -
  KH389_RS25345 (KH389_25345) pilM 5540661..5541512 (-) 852 WP_213606464.1 type IV pilus biogenesis protein PilM -
  KH389_RS25350 (KH389_25350) - 5541687..5544119 (+) 2433 WP_173925097.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 426 a.a.        Molecular weight: 45595.40 Da        Isoelectric Point: 5.2011

>NTDB_id=565639 KH389_RS25330 WP_420831945.1 5537936..5539216(-) (pilQ) [Pseudomonas qingdaonensis strain S-1]
MNRMFRLCAVLMVALPVAGAGLGAEGAGYGGESLSLSFQDVEVRSVLKVLADFTGVNLVASDAVQGKVTLRLHEVPWEQA
LDLVLRSKGLGRRQEGNVLLVAPLAELAGHDTLTRAAQAPPTLVRELLTVSHARAGDIVQLFQAMVEDDDPGAITVDERT
NTIIASQPSARLAYLRRLLAQLDVPVRQVMIEARIVEANVDYEKSLGVRWGGAVQLGGGLRAGGLAADGLPFVDLGVERA
TSGIGLGLVRDSVLLDLELSAMEKTGNGEIISQPKVVTADKETARILKGTEVPYQESGANGTTSTTFREASLSLEVTPQI
TADNRVIMTVKVTKDEPDYLNALNSVPPIRKNEVNAKVQVGDGETIVIGGVYATVQSNVVDKVPFFGDVPYVGRLFRRAV
IQEKKSELLVFLTPRIMSDRAIAVSR

Nucleotide


Download         Length: 1281 bp        

>NTDB_id=565639 KH389_RS25330 WP_420831945.1 5537936..5539216(-) (pilQ) [Pseudomonas qingdaonensis strain S-1]
ATGAACAGGATGTTCCGCCTGTGTGCGGTACTGATGGTGGCGCTGCCGGTAGCAGGTGCAGGGCTTGGGGCCGAGGGGGC
AGGGTATGGCGGCGAGTCGCTGTCGTTGAGTTTCCAGGATGTCGAGGTGCGTTCGGTGCTCAAGGTGCTGGCCGACTTCA
CCGGGGTCAACCTGGTGGCCAGTGATGCAGTACAGGGCAAGGTGACCCTGCGCCTGCACGAGGTGCCCTGGGAACAGGCA
CTGGACCTGGTGCTGCGCAGCAAGGGCCTGGGTCGGCGTCAGGAGGGCAACGTGCTGCTGGTGGCGCCTTTGGCCGAACT
GGCCGGGCACGATACCCTGACCCGTGCTGCGCAGGCCCCGCCCACGCTGGTGCGCGAACTGCTCACGGTCAGCCATGCGC
GGGCCGGGGACATCGTGCAGCTGTTCCAGGCCATGGTCGAGGACGACGACCCCGGCGCGATCACGGTCGACGAGCGCACC
AACACCATCATTGCCAGCCAGCCGTCGGCACGCCTGGCCTACCTGCGGCGGCTGCTCGCGCAGCTCGATGTACCGGTTCG
CCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAATGTCGATTACGAAAAAAGCCTGGGGGTGCGCTGGGGCGGGGCGG
TGCAACTGGGCGGCGGCCTGCGCGCAGGCGGGTTGGCCGCCGACGGGCTGCCGTTCGTCGACCTGGGGGTGGAACGGGCC
ACCTCGGGTATCGGCCTGGGGTTGGTGCGCGACAGTGTGCTGCTCGACCTGGAGCTCAGCGCGATGGAAAAGACCGGCAA
TGGCGAAATCATCTCCCAGCCCAAGGTGGTCACCGCCGACAAGGAAACCGCGCGCATCCTCAAGGGTACCGAGGTGCCGT
ATCAGGAGTCGGGGGCCAACGGCACTACGTCCACCACCTTTCGCGAGGCCTCGCTGTCGCTGGAGGTGACCCCGCAGATC
ACGGCCGACAACCGGGTGATCATGACGGTCAAGGTCACCAAGGACGAGCCGGACTACCTCAATGCCCTCAACAGCGTGCC
GCCGATCCGCAAGAACGAGGTGAATGCCAAGGTTCAGGTGGGGGATGGCGAGACCATCGTGATCGGCGGGGTGTATGCCA
CCGTGCAAAGTAATGTTGTCGACAAGGTGCCATTTTTCGGCGATGTGCCGTATGTTGGGCGGCTGTTTCGTCGCGCTGTC
ATCCAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCGGGCGATTGCTGTGAGTCGTTG
A

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II and III secretion system protein (Secretin)

(261-417)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.817

100

0.615
  pilQ Acinetobacter baumannii strain A118

40.781

100

0.441
  pilQ Acinetobacter baumannii D1279779

40.781

100

0.441
  comQ Acinetobacter baylyi ADP1

41.387

100

0.434
  pilQ Legionella pneumophila strain ERS1305867

38.288

100

0.399
  pilQ Legionella pneumophila strain Lp02

38.288

100

0.399
  pilQ Vibrio campbellii strain DS40M4

38.824

99.765

0.387
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.234

98.122

0.385
  pilQ Vibrio cholerae strain A1552

39.234

98.122

0.385