Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   MKD45_RS04315 Genome accession   NZ_CP092532
Coordinates   894790..896781 (+) Length   663 a.a.
NCBI ID   WP_000098601.1    Uniprot ID   A0A0H3EL60
Organism   Escherichia coli strain DS566-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 889790..901781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKD45_RS04300 (MKD45_04300) - 891876..892607 (+) 732 WP_000758891.1 hypothetical protein -
  MKD45_RS04305 (MKD45_04305) speB 892743..893663 (+) 921 WP_000105562.1 agmatinase -
  MKD45_RS04310 (MKD45_04310) loiP 893754..894512 (-) 759 WP_001298254.1 metalloprotease LoiP -
  MKD45_RS04315 (MKD45_04315) recP/tkt 894790..896781 (+) 1992 WP_000098601.1 transketolase Machinery gene
  MKD45_RS04320 (MKD45_04320) - 897001..897909 (-) 909 WP_000646942.1 prohibitin family protein -
  MKD45_RS04325 (MKD45_04325) cmtB 898285..898728 (+) 444 WP_001236688.1 PTS mannitol transporter subunit IIA -
  MKD45_RS04330 (MKD45_04330) cmtA 898756..900144 (+) 1389 WP_000428818.1 PTS mannitol transporter subunit IICB -
  MKD45_RS04335 (MKD45_04335) yggP 900159..901436 (+) 1278 WP_000853184.1 zinc-binding dehydrogenase -
  MKD45_RS24050 - 901433..901606 (+) 174 Protein_858 fructose-bisphosphatase class II -
  MKD45_RS24055 - 901650..901748 (+) 99 WP_250146959.1 hypothetical protein -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72182.71 Da        Isoelectric Point: 5.5018

>NTDB_id=565602 MKD45_RS04315 WP_000098601.1 894790..896781(+) (recP/tkt) [Escherichia coli strain DS566-2]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQIA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AEQLFEEFGFTVDNVVAKAKALL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=565602 MKD45_RS04315 WP_000098601.1 894790..896781(+) (recP/tkt) [Escherichia coli strain DS566-2]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCACCCGGAAGTGGGTTACACCGC
AGGTGTAGAAACCACCACCGGTCCGCTGGGTCAGGGTATTGCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATCGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCGTTCTACGATGACAACGGTAT
TTCTATCGATGGTCACGTTGAAGGCTGGTTTACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGATATCGACGGTCATGACGCGGCATCCATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCGTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACCCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAGCTGGGCTGGAAATACGCGCCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAGTCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCGAAAGCGAAAGAGTTCATTGCTAA
ACTTCAGGCTAATCCGGCGAAAATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCGTTCGGTCCGCTGTTGCCGG
AATTCCTCGGCGGCTCCGCTGACCTTGCTCCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCAGGTAACTACATCCACTACGGTGTTCGCGAGTTCGGTATGACCGCGATTGCTAACGGTATCTCCCTGCACGGTGGTTT
CCTGCCGTATACCTCAACCTTCCTGATGTTCGTGGAATACGCACGTAATGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGATGGCCCGACTCACCAGCCGGTAGAACAGATCGCT
TCTCTGCGCGTGACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCAGTAGCGTGGAAATACGGCGT
TGAGCGTCAGGACGGCCCGACTGCGCTTATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGTACTGAAGAGCAGCTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGTGCTGGTCAGCCGGAACTGATCTTCATCGCGACCGGTTCTGAA
GTTGAACTGGCTGTTGCCGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTTTCCATGCCGTCTACCGA
CGCATTCGACAAGCAGGACGCGGCTTACCGTGAATCCGTACTGCCGAAAGCGGTTACCGCTCGTGTTGCGGTGGAAGCTG
GCATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGCATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCAGCTGTTTGAAGAGTTCGGCTTCACCGTTGACAACGTTGTTGCGAAAGCAAAAGCGCTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EL60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.024

99.246

0.477