Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LU695_RS18105 Genome accession   NZ_CP092500
Coordinates   3619100..3619540 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain A30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3614100..3624540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU695_RS18085 (LU695_18075) aroP 3615098..3616471 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  LU695_RS18090 (LU695_18080) ampE 3616514..3617368 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  LU695_RS18095 (LU695_18085) ampD 3617365..3617916 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LU695_RS18100 (LU695_18090) nadC 3618004..3618897 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LU695_RS18105 (LU695_18095) pilA 3619100..3619540 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  LU695_RS18110 (LU695_18100) pilB 3619550..3620935 (+) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  LU695_RS18115 (LU695_18105) hofC 3620925..3622127 (+) 1203 WP_096967200.1 protein transport protein HofC -
  LU695_RS18120 (LU695_18110) guaC 3622162..3623205 (-) 1044 WP_001217338.1 GMP reductase -
  LU695_RS18125 (LU695_18115) - 3623361..3623405 (-) 45 WP_120795372.1 protein YacM -
  LU695_RS18130 (LU695_18120) coaE 3623430..3624050 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=565559 LU695_RS18105 WP_000360904.1 3619100..3619540(+) (pilA) [Escherichia coli strain A30]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=565559 LU695_RS18105 WP_000360904.1 3619100..3619540(+) (pilA) [Escherichia coli strain A30]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363