Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KIJ47_RS21050 Genome accession   NZ_CP074584
Coordinates   4421766..4423199 (-) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain L1 delta-T1RM     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4416766..4428199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIJ47_RS21020 (KIJ47_21015) elbB 4418298..4418951 (+) 654 WP_029739612.1 isoprenoid biosynthesis glyoxalase ElbB -
  KIJ47_RS21025 (KIJ47_21020) mtgA 4418948..4419673 (+) 726 WP_029739613.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KIJ47_RS21030 (KIJ47_21025) npr 4419713..4419985 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  KIJ47_RS21035 (KIJ47_21030) rapZ 4419982..4420836 (-) 855 WP_014885397.1 RNase adapter RapZ -
  KIJ47_RS21040 (KIJ47_21035) ptsN 4420882..4421373 (-) 492 WP_029739614.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KIJ47_RS21045 (KIJ47_21040) hpf 4421456..4421743 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  KIJ47_RS21050 (KIJ47_21045) rpoN/rpoN1 4421766..4423199 (-) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  KIJ47_RS21055 (KIJ47_21050) lptB 4423247..4423972 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  KIJ47_RS21060 (KIJ47_21055) lptA 4423979..4424533 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KIJ47_RS21065 (KIJ47_21060) lptC 4424502..4425077 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  KIJ47_RS21070 (KIJ47_21065) kdsC 4425074..4425640 (-) 567 WP_029739615.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KIJ47_RS21075 (KIJ47_21070) kdsD 4425655..4426641 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  KIJ47_RS21080 (KIJ47_21075) - 4426655..4427632 (-) 978 WP_029739616.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=565463 KIJ47_RS21050 WP_023309360.1 4421766..4423199(-) (rpoN/rpoN1) [Enterobacter asburiae strain L1 delta-T1RM]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=565463 KIJ47_RS21050 WP_023309360.1 4421766..4423199(-) (rpoN/rpoN1) [Enterobacter asburiae strain L1 delta-T1RM]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGGCAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCATAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCTATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTAG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48