Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MJJ13_RS01400 Genome accession   NZ_CP092449
Coordinates   290453..291949 (+) Length   498 a.a.
NCBI ID   WP_033549042.1    Uniprot ID   -
Organism   Escherichia coli isolate TKEC21-17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285453..296949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJJ13_RS01370 (MJJ13_01365) tusA 285700..285945 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  MJJ13_RS01375 (MJJ13_01370) zntA 286047..288245 (-) 2199 WP_000106560.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  MJJ13_RS01380 (MJJ13_01375) yhhN 288319..288945 (-) 627 WP_000964724.1 lysoplasmalogenase -
  MJJ13_RS01385 (MJJ13_01380) yhhM 289086..289445 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  MJJ13_RS01390 (MJJ13_01385) yhhL 289448..289717 (-) 270 WP_001295207.1 DUF1145 family protein -
  MJJ13_RS01395 (MJJ13_01390) rsmD 289707..290303 (-) 597 WP_000743181.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  MJJ13_RS01400 (MJJ13_01395) pilA 290453..291949 (+) 1497 WP_033549042.1 signal recognition particle-docking protein FtsY Machinery gene
  MJJ13_RS01405 (MJJ13_01400) ftsE 291952..292620 (+) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  MJJ13_RS01410 (MJJ13_01405) ftsX 292613..293671 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  MJJ13_RS01415 (MJJ13_01410) rpoH 293916..294770 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  MJJ13_RS01420 (MJJ13_01415) livJ 295041..296144 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  MJJ13_RS01425 (MJJ13_01420) panM 296343..296726 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54647.44 Da        Isoelectric Point: 4.1560

>NTDB_id=565186 MJJ13_RS01400 WP_033549042.1 290453..291949(+) (pilA) [Escherichia coli isolate TKEC21-17]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAGVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQTL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=565186 MJJ13_RS01400 WP_033549042.1 290453..291949(+) (pilA) [Escherichia coli isolate TKEC21-17]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAATCGGTTGTAGAAGAAATCGTTCAGGTGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGGAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGACGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378