Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MID01_RS13535 Genome accession   NZ_CP092369
Coordinates   2603168..2603578 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain ZW     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2598168..2608578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID01_RS13505 (MID01_13505) - 2598255..2598395 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MID01_RS13510 (MID01_13510) yqeF 2598700..2599431 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  MID01_RS13515 (MID01_13515) cwlH 2599683..2600435 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MID01_RS13520 (MID01_13520) yqeD 2600622..2601248 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  MID01_RS13525 (MID01_13525) gnd 2601268..2602161 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MID01_RS13530 (MID01_13530) yqeB 2602413..2603135 (+) 723 WP_032726205.1 hypothetical protein -
  MID01_RS13535 (MID01_13535) nucA/comI 2603168..2603578 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MID01_RS13540 (MID01_13540) - 2603774..2604121 (+) 348 Protein_2628 sigma-70 family RNA polymerase sigma factor -
  MID01_RS13545 (MID01_13545) spoIVCA 2604152..2605612 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MID01_RS13550 (MID01_13550) - 2605570..2605748 (-) 179 Protein_2630 hypothetical protein -
  MID01_RS13555 (MID01_13555) arsC 2606103..2606522 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MID01_RS13560 (MID01_13560) acr3 2606534..2607574 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  MID01_RS13565 (MID01_13565) yqcK 2607597..2608037 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MID01_RS13570 (MID01_13570) arsR 2608096..2608413 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=565041 MID01_RS13535 WP_009967785.1 2603168..2603578(-) (nucA/comI) [Bacillus subtilis strain ZW]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=565041 MID01_RS13535 WP_009967785.1 2603168..2603578(-) (nucA/comI) [Bacillus subtilis strain ZW]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529