Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LILO_RS10630 Genome accession   NC_020450
Coordinates   2217856..2219217 (-) Length   453 a.a.
NCBI ID   WP_015427149.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis IO-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2212856..2224217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LILO_RS10595 (lilo_2044) - 2213402..2213896 (+) 495 WP_015427142.1 GNAT family N-acetyltransferase -
  LILO_RS10600 (lilo_2045) - 2213989..2214312 (-) 324 WP_015427143.1 DUF1048 domain-containing protein -
  LILO_RS10605 (lilo_2046) - 2214299..2214646 (-) 348 WP_015427144.1 DUF1048 domain-containing protein -
  LILO_RS10610 (lilo_2047) - 2214633..2214995 (-) 363 WP_015427145.1 PadR family transcriptional regulator -
  LILO_RS10615 (lilo_2048) - 2215116..2215745 (-) 630 WP_015427146.1 hypothetical protein -
  LILO_RS10620 (lilo_2049) - 2215810..2216895 (-) 1086 WP_015427147.1 PIN/TRAM domain-containing protein -
  LILO_RS10625 (lilo_2050) - 2217126..2217812 (+) 687 WP_015427148.1 YoaK family protein -
  LILO_RS10630 (lilo_2051) radA 2217856..2219217 (-) 1362 WP_015427149.1 DNA repair protein RadA Machinery gene
  LILO_RS10635 (lilo_2052) - 2219379..2219606 (-) 228 WP_015427150.1 heavy-metal-associated domain-containing protein -
  LILO_RS10650 (lilo_2053) rplQ 2220422..2220802 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  LILO_RS10655 (lilo_2054) - 2220821..2221759 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  LILO_RS10660 (lilo_2055) rpsK 2221807..2222190 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  LILO_RS10665 (lilo_2056) rpsM 2222211..2222576 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  LILO_RS10670 (lilo_2057) rpmJ 2222594..2222710 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  LILO_RS10675 (lilo_2058) infA 2222764..2222982 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  LILO_RS10680 (lilo_2059) - 2223279..2223926 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49907.31 Da        Isoelectric Point: 5.1748

>NTDB_id=56486 LILO_RS10630 WP_015427149.1 2217856..2219217(-) (radA) [Lactococcus lactis subsp. lactis IO-1]
MAKKKSSFICQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=56486 LILO_RS10630 WP_015427149.1 2217856..2219217(-) (radA) [Lactococcus lactis subsp. lactis IO-1]
ATGGCGAAGAAAAAATCATCATTTATTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTTGTTCCTGGTTCATTAGTTTTGATTGGGGGAGACCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGTCGTGTGCTGTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGCTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACCTTTATTGTTGGTCATG
TGACTAAAGAAGGTCAGCTGGCTGGGCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCGACTGGATCTGCGATTG
TTTGTGCACTTGAGGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACTACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACAGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCAGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGGTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623

Multiple sequence alignment