Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHW13_RS01660 Genome accession   NZ_CP074411
Coordinates   352260..354365 (+) Length   701 a.a.
NCBI ID   WP_024665233.1    Uniprot ID   -
Organism   Pseudomonas syringae strain Susan762     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347260..359365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW13_RS01640 (KHW13_01640) pilM 349423..350487 (+) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  KHW13_RS01645 (KHW13_01645) - 350487..351068 (+) 582 WP_003316651.1 PilN domain-containing protein -
  KHW13_RS01650 (KHW13_01650) pilO 351065..351688 (+) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  KHW13_RS01655 (KHW13_01655) pilP 351685..352212 (+) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  KHW13_RS01660 (KHW13_01660) pilQ 352260..354365 (+) 2106 WP_024665233.1 type IV pilus secretin PilQ Machinery gene
  KHW13_RS01665 (KHW13_01665) aroK 354370..354888 (+) 519 WP_003341387.1 shikimate kinase AroK -
  KHW13_RS01670 (KHW13_01670) aroB 355004..356107 (+) 1104 WP_003422530.1 3-dehydroquinate synthase -
  KHW13_RS01675 (KHW13_01675) - 356118..357734 (+) 1617 WP_003316656.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75454.84 Da        Isoelectric Point: 5.1826

>NTDB_id=564705 KHW13_RS01660 WP_024665233.1 352260..354365(+) (pilQ) [Pseudomonas syringae strain Susan762]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGSGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=564705 KHW13_RS01660 WP_024665233.1 352260..354365(+) (pilQ) [Pseudomonas syringae strain Susan762]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGCGATCGCATCGAACTGAAGCTGGCATTCGATGCCCCGGTGCCTGCACCGCGCG
GTTACACCACGTCGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAT
CTGGGCAGCGGCAATGCGCGCAGTGTGGTTGTGGTACAGGCTCAGGACCGTACTCGGGTGATCATCAACCTGACCACGCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTCGCGGTGCCGCCGCCCACGCCGGCATCCGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACTCAACTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACGATCAGCGTGCGTCCATTGAC
CAACGAGGACGTCGACCGCCGCGCCGCTGACAAACCGGCGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGTGGTATC
ACTCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGCTCGATCACGGTGGACGACCGAACCAACAACATCATTGCCTACCA
GACCCAGGAACGGCTCGACGAGCTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTTCGCTGGGGCGGCTCTACGAACACCAGCGGTAACAGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTT
CGTGGACCTTGGCGCAGCCGGCGCCACCTCGGGTATCGGCCTTGGTTTCGTGACCAACAACACGTTGCTGGACCTTGAAC
TGAGTGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGTGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCTGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.056

100

0.75
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.405
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.061

98.573

0.375
  pilQ Legionella pneumophila strain Lp02

38.026

98.288

0.374