Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   KHW13_RS01615 Genome accession   NZ_CP074411
Coordinates   340166..342385 (-) Length   739 a.a.
NCBI ID   WP_058445363.1    Uniprot ID   -
Organism   Pseudomonas syringae strain Susan762     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 335166..347385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW13_RS01595 (KHW13_01595) hslU 335285..336622 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  KHW13_RS01600 (KHW13_01600) hslV 336689..337219 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  KHW13_RS01605 (KHW13_01605) - 337635..338330 (-) 696 WP_004414617.1 SPOR domain-containing protein -
  KHW13_RS01610 (KHW13_01610) argS 338332..340068 (-) 1737 WP_213567935.1 arginine--tRNA ligase -
  KHW13_RS01615 (KHW13_01615) priA 340166..342385 (-) 2220 WP_058445363.1 primosomal protein N' Machinery gene
  KHW13_RS01620 (KHW13_01620) rpmE 342555..342776 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  KHW13_RS01625 (KHW13_01625) - 342790..343593 (+) 804 WP_213567936.1 thermonuclease family protein -
  KHW13_RS01630 (KHW13_01630) - 343919..345187 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81857.15 Da        Isoelectric Point: 8.4231

>NTDB_id=564703 KHW13_RS01615 WP_058445363.1 340166..342385(-) (priA) [Pseudomonas syringae strain Susan762]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAVRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=564703 KHW13_RS01615 WP_058445363.1 340166..342385(-) (priA) [Pseudomonas syringae strain Susan762]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCCGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTACT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCACCCAAGCAGCGCGAGGCCCTGACCACCCTGGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
ACCCAGCGCACGCCATGAGCACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGG
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTTCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCCCTGGAAAGCCTGCACAACGCTTACACCG
GCCGCTACGGTCTGCTGCGCCTGAATGAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAAAGC
CGACCTCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCCTGGT
GTTTCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAGCTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGTCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATTCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTAATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCATAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
AGTCGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GTGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCACCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48